A rapid, sensitive and selective method for simultaneous determination of seven anticoagulant rodenticide residues (warfarin, coumatetralyl, diphacinone, chlorophacinone, brodifacoum, bromadiolone, and flocoumafen) in human serum by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) has been developed and validated. Serum sample preparation was carried out rapidly and effectively by one-step protein precipitation and analytes extraction using methanol containing 10% acetone.Chromatographic separation was achieved within 10 min using a BEH C18 column (1.7 mm, 2.1 mm  100 mm) and gradient elution with the mobile phase of 5 mM ammonium acetate aqueous solutionmethanol. Good linearity was achieved over three orders of magnitude with a correlation coefficient (r 2 ) of 0.9924-0.9994. The limits of detection for the seven rodenticides ranged from 0.06 mg L À1 (flocoumafen) to 1.5 mg L À1 (diphacinone). The intra-and inter-day relative standard deviations (RSDs) of the seven analytes at the three spiked levels (1  LOQ, 5  LOQ and 10  LOQ) were in the range of 0.6-10.3% and 5.1-15.1%, respectively. The mean recoveries for the seven analytes at the three spiked levels (1  LOQ, 5  LOQ and 10  LOQ) were in the range of 77.3-98.2% with RSDs of 0.58-11.1%. The proposed method was successfully applied for the analysis of the seven anticoagulant rodenticides in human serum.
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