An experimental methodology is presented to measure the temperature variation in cells with the usage of CdTe/CdS/ZnS core/shell/shell quantum dots as nanothermometers.
This
paper discusses synthesis and application of dual functional
SiO2@Au@SiO2@QD composite nanoparticles for
integrated intracellular heating with temperature motoring. The particles
are of multilayered concentric structure, consisting of Au nanoshells
covered with quantum dots, with the former for infrared heating through
localized surface plasma resonance while the later for temperature
monitoring. The key to integrate plasmonic-heating/thermal-monitoring
on a single composite nanoparticle is to ensure that the quantum dots
be separated at a certain distance away from the Au shell surface
in order to ensure a detectable quantum yield. Direct attachment of
the quantum dots onto the Au shell would render the quantum dots practically
functionless for temperature monitoring. To integrate quantum dots
into Au nanoshells, a quantum quenching barrier of SiO2 was created by modifying a Stöber-like process. Materials,
optical and thermal characterization was made of these composite nanoparticles.
Cellular uptake of the nanoparticles was discussed. Experiments were
performed on simultaneous in vitro heating and temperature
monitoring in a cell internalized with the dual-functional SiO2@Au@SiO2@QD composite nanoparticles.
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