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Objectives Abnormal expressions of microRNAs (miRNAs) are demonstrated in pancreatic cancer (PaC), but a major part of the mechanism remains elusive. This study mainly aimed to structure a coexpressed network of long noncoding RNA (lncRNA) and messenger RNA (mRNA) in PaC, as well as to explore their direct targets. Methods LncRNA and mRNA microarrays were used to determine the expression profiles in PaC cells. Analysis of Kyoto Encyclopedia of Genes and Genomes pathway was performed to identify pathways associated with differentially expressed mRNAs. Coexpression profiles were identified by constructing differentially expressed lncRNA‐mRNA regulatory network and further validated by quantitative real‐time polymerase chain reaction assay and western blot assay. The bioinformatics computational method was applied to predict the biological target of lncRNA and mRNA, which was identified by luciferase reporter assay. Migration/invasion ability and apoptosis rate of cells were assessed by transwell assay and flow cytometry assay. Results It was identified that the level of urothelial cancer associated 1 (UCA1) was increased in PaC cells, and the inhibition of UCA1 suppressed migration and invasion ability of the cancer cells. The luciferase reporter assay recognized that miR‐107 was targeted by UCA1, and integrin subunit α 2 (ITGA2) was further targeted by miR‐107. This confirmed the prediction of lncRNA‐miRNA‐mRNA regulation mechanism. In the regulatory pathways, UCA1 and ITGA2 promoted PaC progression via focal adhesion pathway related proteins such as ITGA3, SRC protooncogene/nonreceptor tyrosine kinase, protein tyrosine kinase 2, and AKT serine/threonine kinase 1. Conclusion The study revealed a regulatory network of UCA1‐miR‐107‐ITGA2 and validated UCA1 and ITGA2 as potential prognostic factors for PaC.

Combined hepatocellular-cholangiocarcinoma (CHCC) is a rare type of primary liver cancer (PLC). The aim of this study was to investigate the disease characteristics in CHCC patients and compare them with those in hepatocellular carcinoma (HCC) or intrahepatic cholangiocarcinoma (ICC).The perioperative and follow-up data of CHCC patients (n = 15), HCC patients (n = 577), and ICC patients (n = 61) were retrospectively analyzed, and the clinicopathological characteristics were compared among these 3 groups.In the CHCC group, the serum level of AFP was significantly higher than that of the ICC group (P = .002), and the CA19-9 level was higher than that of the HCC group (P = .011). The positive rates of CK7 and CK19 expression were higher in CHCC group than in HCC group (both P < .001), while the positive rates of Glypican–3 and Hepatocyte expression were higher in CHCC group than in ICC group (both P < .001). Meanwhile, the CHCC patients were likely to have undergone more MJH/LT than the HCC patients (P = .037) and the ICC patients (P = .011). Macrovascular invasion and lymph node metastasis in the CHCC group were significantly higher but satellite lesions were similar, compared to the HCC group. Both the 1-year disease-free survival (DFS) and the 1-year overall survival (OS) for the CHCC patients were worse than those for the HCC patients. AFP ≥ 400 ng/ml, tumor size ≥5 cm, tumor number ≥2, macro- and microvascular invasion, distant metastasis and positive margin were risk factors for both DFS and OS for the PLC patients. Multivariate analysis also confirmed that ICC and lymph node metastasis were risk factors for DFS and MJH/LT was risk factor for OS.CHCC patients appear to have intermediate clinical characteristics in comparison with the HCC and ICC patients, and the 1-year DFS and OS for the CHCC patients was worse than the HCC patients, but similar to the ICC patients.

The precise tuning of the active layer morphology to improve organic solar cells (OSCs) efficiency remains a key issue in the field of organic photovoltaics. Herein, a new solution to the above problem is provided by using the dual‐solvent modulated polymer‐assisted sequential spin‐coating method. Herein, the sequential spin‐coated OSCs based on the D18‐Cl/Y6 system are prepared for the first time and an efficiency of 16.38% is obtained, similar to that of bulk heterojunction OSCs. On this basis, the performance is further improved by using a dual solvent to balance the dissolution and crystallization of D18‐Cl, separately optimizing the morphology of the donor layer and allowing the subsequent spin‐coated Y6 solution to penetrate uniformly into the D18‐Cl framework. After the dual‐solvent treatment, the D18‐Cl (CF + CB)/Y6‐based device obtains a power conversion efficiency (PCE) of 17.33% and the D18‐Cl (THF + CB)/Y6‐based device achieves an even better PCE of 17.73%. It is worth noting that no post‐treatment is adopted here and after 2500 h of placement, the efficiency of the aforementioned devices is still 90% of the original. Thus, this work provides a simple method for tuning the film morphology to prepare efficient and stable devices, which is beneficial for future commercial production of OSCs.

For optimizing the performance of photovoltaic devices, fabricating ternary organic solar cells (TOSCs) is a common strategy. However, the hybrid system involves complex intermolecular interactions, which poses a challenge to...