ObjectivePeriodontitis is characterized by alveolar bone injury and absorption, with high incidence and poor treatment effect. Proliferation, migration, differentiation and apoptosis of osteoblasts are identified as key factors during the regeneration of alveolar bone tissue processes. Periodontal ligament stem cells (PDLSCs) have been proved to be a possible candidate for the treatment of periodontitis due to its multiple advantages, such as increasing the regenerative capacity of bone tissue. However, the effect of exosomes derived from PDLSCs (PDLSC‐Exo) on osteoblasts remains to be further studied.Methods and MaterialsIn this work, cell proliferation, migration, osteogenic differentiation, and H2O2‐induced apoptosis were detected after cells were exposed to PDLSC‐Exo by CCK‐8, scratch wound assay, alizarin red S and alkaline phosphatase staining, real‐time PCR, flow cytometry, tunel assay, and so on. Moreover, the activation of PI3K/AKT and MEK/ERK signaling pathways was evaluated by western blotting.ResultsWe found that PDLSC‐Exo are capable of promoting hFOB1.19 cell proliferation, migration and osteogenic differentiation, inhibiting H2O2‐induced apoptosis, and activating the PI3K/AKT and MEK/ERK signaling pathways.ConclusionThese results suggest that PDLSC‐Exo may be a promising therapeutic for osteoblastic damage.
China’s highway construction has moved from “construction-oriented” to the development stage of “equal attention to construction and maintenance”. The infrastructure represented by urban expressways, under heavy and complex traffic loads, is prone to a variety of diseases that take place in the middle and lower layers of pavement such as “frost boils” and “voids”, which cannot be repaired ideally with the help of conventional detection and treatment techniques. In order to solve the above problems, this paper adopts nondestructive testing plans such as ground penetrating radar and falling weight deflectometer to conduct multidimensional rapid detection of the road surface to obtain the image information and mechanical data of the road structure. Based on the improved calculation method, the pavement disease area, depth and type can be effectively judged. Combined with the observation of water level, the polymer grouting reinforcement plan is designed to eliminate the problems in the middle and lower layers of the road surface. It can effectively reduce the incidence of diseases in the upper layer of the sidewalk, and significantly improve the efficiency and service level of the sidewalk.
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