The objective of this experiment was to test the effects of supplementation of defatted black soldier fly (Hermetia illucens) larvae (BSFL) meal in beagle dogs. A total of nine healthy female beagles (initial body weight 12.1 ± 1.76 kg) were fed grain-based diets with three levels of BSFL meal (0, 1% or 2%) in a 42-day feeding trial. At the end of week 6 of the experiment, all dogs were intraperitoneally challenged with Escherichia coli lipopolysaccharide (LPS) at 100 μg/kg of body weight. Albumin concentration was linearly increased with increasing BSFL meal level (P<0.05). A linear increase (P<0.05) in calcium concentration was observed when increasing dietary BSFL meal. Although dietary treatments did not affect the digestibility of ether extract, the digestibility of dry matter and crude protein were linearly increased with increasing the level of BSFL meal. The concentration of tumor necrosis factor-α was linearly decreased but glutathione peroxidase (GPx) concentration was linearly increased when increasing the level of BSFL meal at 6 h after challenge (P<0.05). In addition, there were quadratic increases in concentrations of GPx and superoxide dismutase with increasing dietary BSFL meal level at 3 h after challenge (P<0.05). These findings from the present study demonstrate that BSFL meal can be supplemented in the diet to convert beneficial effects to beagle dogs, indicated as improved digestibility of dry matter and crude protein and anti-inflammatory and anti-oxidative capacity.
Effects of adding protease with or without fructooligosaccharide (FOS) to low protein diet on growth performance, nutrient digestibility and fecal noxious gas emission were evaluated in 160 finishing pigs (57.70 ± 1.16 kg) in a 9-week study. Pigs were randomly divided into four dietary treatments, PC: positive control diet (15.97% crude protein (CP)); NC: negative control diet (12.94% CP); PRO: NC supplemented with 0.05% protease; PROFOS: NC supplemented with 0.05% protease and 0.1% FOS. During weeks 4-9 and weeks 0-9, gain : feed ratio was impaired (P < 0.05) in pigs fed NC diet compared with those fed PC, PRO and PROFOS diets. Pigs fed PC, PRO and PROFOS diets had higher (P < 0.05) apparent total tract digestibility (ATTD) of CP than pigs fed NC diet. Pigs fed PROFOS diet had reduced (P < 0.05) ammonia emissions compared to pigs fed NC and PRO diets. These data indicate that reducing dietary CP concentrations impaired growth performance, decreased ATTD of CP and reduced ammonia emissions. Supplementation of protease in low CP diet improved growth performance and increased ATTD of CP. Dietary supplementation with protease and FOS in low CP diet improved growth performance, increased ATTD of CP and decreased fecal ammonia emission.
The aim of this study was to evaluate the effects of dietary levels of xylanase on production performance, egg quality, nutrient digestibility, and excreta microbiota shedding of laying hens in a 12-week trial. Two-hundred-forty Hy-Line brown laying hens (44 wk old) were distributed according to a randomized block experimental design into one of 4 dietary treatments with 10 replicates of 6 birds each. The 4 dietary treatments were corn-soybean-meal-wheat-based diets supplemented with 0, 225, 450, or 900 U/kg xylanase. Daily feed intake, egg production, egg weight, egg mass, feed conversion ratio, and damaged egg rate showed no significant response to increasing xylanase supplementation during any phase (P > 0.05). No significant responses were observed for apparent total tract digestibility of dry matter, nitrogen, or gross energy (P > 0.05). A significant linear increase to increasing xylanase supplementation was seen for lactic acid bacteria numbers, although coliforms and Salmonella counts were not affected. Increasing the dietary xylanase resulted in a significant linear increase in eggshell thickness in wk 3, 6, 9, and 12 (P < 0.05). In addition, a significant linear increase occurred for Haugh unit and albumen height in wk 12 (P < 0.05). In summary, the inclusion of xylanase in corn-soybean-meal-wheat-based diets increased eggshell thickness, Haugh unit, albumen height, and excreta lactic acid bacteria count but had no effect on production performance or nutrient digestibility.
The present experiment was conducted to determine effects of herbs (Artemisia capillaris and Acanthopanax senticosus) in natural and fermented forms on growth performance, nutrient digestibility, blood parameters, meat quality and fatty acid composition in growing-finishing pigs. A total of 96 pigs [(Landrace  Yorkshire)  Duroc] with an average initial body weight (BW) of 25.46 ± 1.07 kg were randomly allotted into one of three dietary treatments. The dietary treatments included: (1) CON (basal diet), (2) NH (basal diet þ0.05% natural herbs) and (3) FH (basal diet þ0.05% fermented herbs). Pigs fed NH and FH diets had greater final BW than those fed CON diet (p < .05). During the whole period of the experiment, pigs fed NH and FH diets had a greater average daily gain than those fed CON diet, and the average daily feed intake in FH dietary treatment was greater than CON dietary treatment (p < .05). The FH dietary treatment had improved apparent total tract digestibility (ATTD) of dry matter compared with CON and NH dietary treatments (p < .05). The ATTD of nitrogen in NH and FH dietary treatments was greater than that in CON dietary treatment (p < .05). Moreover, fermented herbs decreased saturated fatty acids (SFA) but increased polyunsaturated fatty acids (PUFA) and PUFA to SFA ratio in Longissimus dorsi muscle. In conclusion, natural or fermented herbs improved growth performance and nutrient digestibility in growing-finishing pigs. Additionally, fermented herbs supplementation positively changed fatty acid profiles in Longissimus dorsi muscle.
This study was to investigate the effects of Epigallocatechin‐3‐gallate (EGCG) on intestinal morphology, antioxidant capacity and anti‐inflammatory response in heat‐stressed broiler. A total of 192 2‐week‐old Arbour Acres broilers chickens were divided into four groups with six replicates per group and eight chickens per replicate: one thermoneutral control group (28°C, group TN), which was fed the basal diet; and three cyclic high‐temperature groups (35°C from 7:00 to 19:00 hr; 28°C from 19:00 hr to 7:00 hr, heat stress group), which were fed the basal diet supplementation with EGCG 0 mg/kg (group HS0), 300 mg/kg (group HS300) and 600 mg/kg (group HS600). The gut morphology and intestinal mucosal oxidative stress indicators, as well as intestinal barrier‐related gene expression, were analysed. The results showed that compared with group TN, heat stress reduced the villus height (VH), activities of glutathione peroxidase (GSH‐Px), superoxide dismutase (SOD)and catalase (CAT), increased the crypt depth (CD) and malondialdehyde (MDA)content at 21, 28 and 35 days (p < 0.05). After the heat‐stressed broilers were supplemented with EGCG, VH, VH/CD (V/C), and the activities of GSH‐Px, SOD and CAT were increased, and CD and MDA content were reduced compared with those in group HS0 without EGCG supplementation at 21, 28 and 35 days (p < 0.05). The EGCG supplementation promoted the gene expression of nuclear factor‐erythroid 2‐related factor 2 (Nrf2), Claudin‐1, Mucin 2 (Muc2) and alleviated the nuclear factor‐kappa B (NF‐κB) and lipopolysaccharide‐induced tumour necrosis factor (LITAF) gene expression compared with group HS0 (p < 0.05). Moreover, intestinal morphology was strongly correlated with antioxidant ability and inflammatory response. In conclusion, EGCG alleviated the gut oxidative injury of heat‐stressed broilers by enhancing antioxidant capacity and inhibiting inflammatory response.
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