Variations in maternal behavior induce long-lasting effects on behavioral and neuroendocrine responses to stress. The aim of this study was to analyze developmental parameters, reproductive function, and anxiety-related behaviors of male and female rats raised by mothers that naturally display high and low levels of maternal licking behavior. Results showed that an increase in licking behavior received by the pups accelerated their eye opening and reduced fear behavior assessed in the open field test. Additionally, female offspring of high licking (HL) mothers showed decreased ovulation and lordosis intensity. In contrast, males from HL and low licking (LL) mothers did not differ in their reproductive function, suggesting a gender difference in maternal effects. Present results showed that individual differences in maternal behavior appear not only to be predictive of later emotionality and stress-responsivity in the offspring, but can also modulate the reproductive function of females. Maternal genetic factors, differences in the prenatal intrauterine milieu, or a combination of these cannot be excluded to explain the effects observed.

No abstract

In rats, mating at postpartum estrus and delayed dispersal of the young would result in the overlapping of two different-age litters. As a consequence, newborn pups' early experience will include not only that acquired during the interaction with the mother and age-matched littermates, but also with older siblings. As early-life experience modulates rodents' brain function, behavior and reproduction, we aimed to assess how changes in the early environment provoked by the overlapping of litters would affect emotionality, stress response and reproductive functions of male and female pups during adulthood. Results showed that both male and female overlapped reared pups exhibited a reduced behavioral inhibition in the open field test during adulthood. In addition, overlapped reared adult females, but not males, showed a blunted corticosterone response to an acute stressor during diestrus and a reduction in sexual behavior. In summary, natural changes in early experience provoked by the overlapping of litters, long-term modulate affective and reproductive behaviors, and the endocrine stress response in a sex dimorphic manner.