The unmanned aerial vehicle (UAV) sensors and platforms nowadays are being used in almost every application (e.g., agriculture, forestry, and mining) that needs observed information from the top or oblique views. While they intend to be a general remote sensing (RS) tool, the relevant RS data processing and analysis methods are still largely ad-hoc to applications. Although the obvious advantages of UAV data are their high spatial resolution and flexibility in acquisition and sensor integration, there is in general a lack of systematic analysis on how these characteristics alter solutions for typical RS tasks such as land-cover classification, change detection, and thematic mapping. For instance, the ultra-high-resolution data (less than 10 cm of Ground Sampling Distance (GSD)) bring more unwanted classes of objects (e.g., pedestrian and cars) in land-cover classification; the often available 3D data generated from photogrammetric images call for more advanced techniques for geometric and spectral analysis. In this paper, we perform a critical review on RS tasks that involve UAV data and their derived products as their main sources including raw perspective images, digital surface models, and orthophotos. In particular, we focus on solutions that address the “new” aspects of the UAV data including (1) ultra-high resolution; (2) availability of coherent geometric and spectral data; and (3) capability of simultaneously using multi-sensor data for fusion. Based on these solutions, we provide a brief summary of existing examples of UAV-based RS in agricultural, environmental, urban, and hazards assessment applications, etc., and by discussing their practical potentials, we share our views in their future research directions and draw conclusive remarks.
Background/Aims: MicroRNAs (miRNAs, miRs) have emerged as critical regulators of cancer cell proliferation. The effect of miR-221 on cancer cell growth could be significantly changeable in different cell lines. Although miR-221 was reported to promote the cell growth of pancreatic ductal adenocarcinoma (PDAC) cells, its role in Capan-2 cell line is largely unknown. Methods: Capan-2 cells were transfected with miR-221 mimics, inhibitors, or negative controls. Cell Counting Kit-8 was used to determine cell viability. EdU staining and cell cycle analysis were used to measure cell proliferation. Western blotting was used to detect the expression levels of PTEN and phospho-Akt. The PI3K-Akt pathway activator SC-79 and inhibitor LY294002 were used to perform the rescue experiment in determining cell proliferation. Results: Overexpressing miR-221 significantly increased cell vitality and promoted cell proliferation and G1-to-S phase transition of the cell cycle in Capan-2 cells, while inhibition of miR-221 decreased that. The protein level of PTEN in Capan-2 cells was downregulated by overexpressing miR-221, while upregulated by inhibiting miR-221. Consistently, enhanced phosphorylation of AktSer473 was observed in miR-221 overexpressed Capan-2 cells, and the opposite result was found in miR-221 inhibited cells. LY294002 restored the pro-proliferation effect of miR-221 on Capan-2 cells, while SC-79 had no additional effect on cell proliferation in Capan-2 cells transfected with miR-221 mimics. Conclusion: Our study indicates that miR-221 is an oncogenic miRNA which promotes Capan-2 cells proliferation by targeting PTEN-Akt pathway.
BackgroundMultiple microRNAs (miRNAs, miRs), including miR-21, have been documented to be critical regulators of liver regeneration, but the mechanism underlying their roles in hepatocyte proliferation and cell cycle progression is still far from understood.Material/MethodsmiR-21 levels were determined using qRT-PCRs in mouse livers at 48 h after 70% partial hepatectomy (PH-48 h). Cell proliferation was determined by use of a cell-counting kit-8 (CCK-8), EdU incorporation staining, and flow cytometry. Phosphatase and tensin homolog (PTEN) expressions were determined using qRT-PCR and Western blot analysis. PTEN siRNA was used to perform the rescue experiment.ResultsA marked upregulation of miR-21 was observed in mouse livers at 48 h after 70% partial hepatectomy (PH-48 h) compared to 0 h after PH (PH-0 h). Overexpression of miR-21 was associated with increased proliferation and a rapid G1-to-S phase transition of the cell cycle in BNL CL.2 normal liver cells in vitro. In addition, we showed that PTEN expression was inversely correlated with miR-21 in BNL CL.2 cells and demonstrated that PTEN expression is lower in mouse livers at PH-48 h. Moreover, the presence of PTEN siRNA significantly abolished the suppressive effect of miR-21 inhibitor on hepatocyte proliferation.ConclusionsmiR-21 overexpression contributes to liver regeneration and hepatocyte proliferation by targeting PTEN. Upregulation of miR-21 might be a useful therapeutic strategy to promote liver regeneration.
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