BACKGROUND Soybean meal (SBM) is the most common protein source used in the poultry and livestock industries. It has high‐quality protein, an excellent amino acid (AA) profile, and positive isoflavone properties. However, the antigen proteins in SBM are unsuitable for young animals. The objective of this study was to identify a Bacillus strain that can degrade soybean antigen proteins, and to evaluate the feasibility of its application in SBM fermentation. RESULTS Bacillus velezensis DP‐2 was isolated from Douchi, a fermented Chinese food. It degraded 96.14% and 66.51% of glycinin and β‐conglycinin, and increased the trichloroacetic acid‐soluble protein (TCAN) content by 5.46 times in the SBM medium. DP‐2 could secrete alkaline protease and neutral protease, with productivities of 5.85 and 5.99 U mL−1. It had broad‐spectrum, antibacterial activities against Rhizopus nigricans HR, Fusarium oxysporum ACCC37404, Penicillium digitatum SQ2, Aspergillus flavus C1, Aspergillus niger ACCC30005, Trichoderma viride YZ1, Candida tropicalis CICC1630, and Salmonella sp. ZY. For SBM fermentation, the optimal inoculum rate, temperature, and fermentation time of DP‐2 were 2.21 × 107 CFU g−1, 37 °C, and 48 h, respectively. The fermented soybean meal (FSBM) was cream‐colored and glutinous. Its crude protein (CP), soluble protein, and TCA‐N content were improved by 13.45%, 12.53%, and 6.37 times, respectively. The glycinin and β‐conglycinin content were reduced by 78.00% and 43.07%, respectively, compared with raw SBM. CONCLUSIONS Bacillus velezensis DP‐2 has potential as a starter culture for SBM fermentation. © 2020 Society of Chemical Industry
The present study investigated the effects of supplementing bioactive peptides derived from rapeseed protein (rapeseed peptide, Rsp) on the growth performance, serum biochemistry and faecal micro‐organism composition of weaned piglets. Sixty Duroc × Landrace × Yorkshire weaned piglets of similar weights were randomly divided into three groups. The control group (NC) was fed a basal diet, and the two treatment groups, Rsp‐1 and Rsp‐2, were fed a basal diet supplemented with 1% or 2% Rsp, respectively, for 28 days. Each treatment consisted of five replicates with four piglets per replicate. The results showed that Rsp treatment significantly improved the average daily gain and had a better feed‐to‐gain ratio (p < 0.05). The diarrhoea incidence and indices of Rsp‐1 and Rsp‐2 groups were significantly lower than the NC group (p < 0.05), and the effect of Rsp‐2 on reducing the incidence of diarrhoea was significantly higher than that of Rsp‐1 (p < 0.05). The serum albumin, serum immunoglobulin A and catalase levels of the Rsp‐1 and Rsp‐2 groups were significantly better than the NC group (p < 0.05). Additionally, Rsp treatment significantly gained the relative abundance of faecal Lactobacillaceae and decreased the relative abundance of faecal Eubacterium_coprostanoligenes_group, Treponema and Coprococcus (p < 0.05). In summary, Rsp supplementation improved the growth performance, ameliorated the diarrhoea, enhanced the immune and antioxidant functions and changed the composition of faecal micro‐organisms in piglets. These findings indicate that Rsp positively affected the health of weaned piglets.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.