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Long non-coding RNA metastasis associated with lung adenocarcinoma transcript 1 (MALAT1) contributes to chemotherapy resistance in some cancers, but the role of MALAT1 in sunitinib (SU) chemoresistance of carcinoma (RCC) is still unknown. In this study, MALAT1 expression in SUresistance tumor tissues and cells was tested by qRT-PCR. Then, CCK-8, Annexin V-FITC/PI, transwell, and Western blotting assays were used to evaluate cell viability and IC50, apoptosis, cell invasion, and resistance of SU-resistance RCC cells after transfected with small interfering RNA against MALAT1. Further, RNA pull-down and luciferase reporter assay were applied to investigate the underlying mechanism of MALAT1 in SU resistance. The results showed that MALAT1 expression was dramatically upregulated in SU-resistance RCC tissues and cell lines. Knockdown of MALAT1 inhibited proliferation, invasion, and SU chemoresistance, but induced apoptosis in RCC cells. The results of RNA pull-down and luciferase reporter assay indicated that MALAT1 could interact with miR-362-3p and miR-362-3p interact with RasGAP SH3-domain-Binding Protein 1 (G3BP1). Moreover, G3BP1 also played a role in SU chemoresistance of RCC cells, and MALAT1 could perform as a miR-362-3p sponge to modulate G3BP1 expression. Rescue experiments suggested that downregulation of miR-362-3p and overexpression of G3BP1 can reverse the SU chemosensitivity of MALAT1 knockdown in RCC cells. In conclusion, depletion of LncRNA MALAT1 inhibited SU chemoresistance through modulating G3BP1 via sponging miR-362-3p in RCC cells, suggesting that targeting MALAT1 may be a potential therapeutic strategy for SU-resistance RCC.

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Power quality problem, because of its various forms and occurrence frequency, has become one of the most critical challenges confronted by a power system. Meanwhile, the development of renewable energy has led to more demands for an integrated system that combines both merits of sustainable energy generation and power quality improvement. In this context, this paper discusses an integrated photovoltaic-unified power quality conditioner (PV-UPQC) and its control strategy. The system is composed of a series compensator, shunt compensator, dc-bus, and photovoltaic array, which conducts an integration of photovoltaic generation and power quality mitigation. The fuzzy adaptive PI controller and the improved Maximum Power Point Tracking (MPPT) technique are proposed to enhance the stability of dc-bus voltage, which is aimed at the power balance and steady operation of the whole system. Additionally, the coordinate control strategy is studied in order to ensure the normal operation and compensation performance of the system under severe voltage sag condition. In comparison to the existing PV-UPQC system, the proposed control method could improve the performance of dc-bus stability and the compensation ability. The dynamic behavior of the integrated system were verified by simulation in MATLAB and PLECS. Selected results are reported to show that the dc-bus voltage was stable and increased under severe situations, which validates the effectiveness of the proposed integrated PV-UPQC system and its control strategy.

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