Background: Osteosarcoma (OS) is the most common primary bone tumor in group of children and adolescents. Increasing studies showed that long non-coding RNAs (lncRNAs) exerted important functions in the development of tumors, including OS. LINC01535 is an lncRNA which has been studied in cervical cancer but not in OS. Aim of the Study: This study was aimed to explore the biological function and mechanism of LINC01535 in OS. Methods: LINC01535 expression was detected by qRT-PCR. Colony formation assay, EdU assay and CCK-8 assay were applied to check cell proliferation ability in OS. Flow cytometry analysis was conducted to measure cell apoptosis capacity. Wound healing assay and transwell assay were performed to assess cell migration and invasion. Luciferase reporter assay and RNA pull-down assay were carried out to verify the molecular mechanism. Results: The high expression of LINC01535 was presented in OS tissues and cell lines compared with adjacent normal tissues and human osteoblasts. Moreover, OS patients with high LINC01535 expression exhibited poor prognosis. Loss-of-function assay revealed that silenced LINC01535 significantly attenuated cell proliferation, migration and invasion, and enhanced cell apoptosis in OS. Through mechanistic exploration, we found that LINC01535 interacted with miR-214-3p, and KCNC4 was validated to be a target gene of miR-214-3p. The levels of KCNC4 mRNA and protein were positively modulated by LINC01535 and reversely mediated by miR-214-3p. Based on rescue experiments, KCNC4 overexpression reserved the suppressive function of silenced LINC01535 on OS cell growth, migration and invasion. Conclusion: LINC01535, miR-214-3p and KCNC4 constituted an effective axis that exerted a pregnant regulation in OS development, which is a quite meaningful discovery for exploring potential therapeutic methods for OS patients.
Objective: The purposes of this study were to prospectively compare the clinical outcomes of distal humeral shaft fractures treated by lateral plating technique and posterior plating technique. Material and methods: From Jan 2017 to Aug 2019, fifty-one patients with distal humeral shaft fractures treated by lateral or posterior plating technique were collected. Patients were divided into two groups: the lateral plating group (group I, 28 patients) and the posterior plating group (group II, 23 patients). There was no statistical difference between the two groups in age, gender, injured arms, injury mechanism, AO/ASIF (Arbeitsgemeinschaft für Osteosynthesefragen/Association for the Study of Internal Fixation ) classification and duration from injury to surgery (P > 0.05). Operation time, perioperative bleeding volume, hospitalization, complication rate were analyzed. Elbow function was assessed by the Mayo Elbow Performance Score (MEPS) and range of flexion and extension motion (ROM) was at last follow-up. Results: The mean duration of follow-up in group I was 14.25±1.818 months (range, 12–20 months), and was 15.26±2.717 months (range, 12–22 months) in group II. There was no significant difference in these items , including operation time, perioperative bleeding volume, AO/ASIF classification and hospitalization between the two groups (P > 0.05). Radiological and clinical evidence of bone healingwas seen in all patients. In group I, the mean union time was 13.00±0.41weeks (from10 to16weeks), the mean elbow ROM was 136.96/5.25° (range, 130-140° and 0-9°, respectively). In group II, the mean union time was 13.30±0.43 weeks (from10 to16 weeks), the mean elbow ROM was 138.52 /4.83° (range, 130-146° and 0-9°, respectively). The mean MEPS of lateral plating was 86.61±1.82 (from70 to100 points) and that of posterior plating was 85.87±1.88 (form70 to100points). No significant differences were found in union time, elbow ROM and MEPS between the two groups (P>0.05). The overall complication rate in group II was found to be higher than group I (P=0.014). Conclusions: Our results presented that both lateral and posterior plating techniques for distal humeral shaft fractures achieved satisfied treatment results; and lateral plating treatment with a lower complication rate .
Objective Establish a new classification system of distal radius fracture based on computed tomographic (CT), and evaluate its reliability and reproducibility preliminarily, and provide a new theoretical reference for clinicians to use the clinical classification system. Methods The imaging data and clinical data of 204 inpatients with distal radius fractures during 6 years from January 1, 2014 to January 1, 2019 in orthopaedic department were analyzed retrospectively and classified based on CT. Three observers evaluated the image data of 48 randomly selected cases based on CT at different time nodes of T1 and T2. Cohen's kappa was used to calculate the consistency. Results Among 204 cases, there were 12 cases of type A, including 6 cases of type A1, 4 cases of type A2 and 2 cases of type A3. There were 6 cases of type B, including 2 cases of type B1, 2 cases of type B2 and 2 cases of type B3. There were 186 cases of type C, including 32 cases of type C1, 127 cases of type C2 and 27 cases of type C3. There was no significant difference in Disabilities of the Arm, Shoulder and Hand (DASH), Patient-Rated Wrist Evaluation(PRWE) and VAS scores among all types (P > 0.05). The results of interobserver reproducibility were kappa = 0.985, ICC = 0.984 in the first evaluation, kappa = 0.986, ICC = 0.986 in the second evaluation. The results of intraobserver reproducibility were O1 = 0.991, O2 = 0.991, O3 = 0.989 respectively. Conclusions The new classification system of distal radius fracture based on CT has theoretical and practical significance for incision selection, fracture reduction and internal fixation. ABC typing system is clear, comprehensive, easy to understand and remember. Moreover, it has higher interobserver reliability and intraobserver reproducibility than other systems reported at present.
Published data on earthquake-associated peripheral nerve injury is very limited. Ultrasonography has been proven to be efficient in the clinic to diagnose peripheral nerve injury. The aim of this study was to assess the role of ultrasound in the evaluation of persistent peripheral nerve injuries 1 year after the Wenchuan earthquake. Thirty-four patients with persistent clinical symptoms and neurologic signs of impaired nerve function were evaluated with sonography prior to surgical repair. Among 34 patients, ultrasonography showed that 48 peripheral nerves were entrapped, and 11 peripheral nerves were disrupted. There was one case of misdiagnosis on ultrasonography. The concordance rate of ultrasonographic findings with those of surgical findings was 98%. A total of 48 involved nerves underwent neurolysis and the symptoms resolved. Only five nerves had scar tissue entrapment. Preoperative and postoperative clinical and ultrasonographic results were concordant, which verified that ultrasonography is useful for preoperative diagnosis and postoperative evaluation of injured peripheral nerves.
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