Luminescent nanomaterials have attracted great attention in luminescence‐based bioanalysis due to their abundant optical and tunable surface physicochemical properties. However, luminescent nanomaterials often suffer from serious autofluorescence and light scattering interference when applied to complex biological samples. Time‐resolved luminescence methodology can efficiently eliminate autofluorescence and light scattering interference by collecting the luminescence signal of a long‐lived probe after the background signals decays completely. Lanthanides have a unique [Xe]4fN electronic configuration and ladder‐like energy states, which endow lanthanide‐doped nanoparticles with many desirable optical properties, such as long luminescence lifetimes, large Stokes/anti‐Stokes shifts, and sharp emission bands. Due to their long luminescence lifetimes, lanthanide‐doped nanoparticles are widely used for high‐sensitive biosensing and high‐contrast bioimaging via time‐resolved luminescence methodology. In this review, recent progress in the development of lanthanide‐doped nanoparticles and their application in time‐resolved biosensing and bioimaging are summarized. At the end of this review, the current challenges and perspectives of lanthanide‐doped nanoparticles for time‐resolved bioapplications are discussed.

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The equilibrium solubilities of two dodecylpolyoxyethylene polyoxypropylene ether nonionic surfactants (A(EO)BmB(PO) n ) in supercritical carbon dioxide were measured at temperatures ranging from (308 to 328) K and pressures from (13.6 to 32.0) MPa in a stainless steel variable-volume view cell. The experimental data were correlated using a semiempirical model. The calculated results show satisfactory agreement with the experimental data.

Two‐dimensional (2D) nanoflakes represent an appealing class of materials for optoelectronics applications due to their unique layered structures and excellent electronic properties. However, the lack of easy‐to‐manipulate and effective methods for large‐scale production of these 2D materials limits their potential for applications. Also, few efforts have been made to explore their applications in biological fields. This work reports the preparation of large quantities of 2D In2Se3 nanosheets through a solvent exfoliation technique. Transmission electron microscopy and atomic force microscopy results show that the In2Se3 nanosheets are obtained with lateral sizes of tens of nanometers to hundreds of nanometers and thickness of 2–17 layers. Raman features coupled with the X‐ray diffractometry results unequivocally confirm the as‐prepared In2Se3 nanosheets to be α phase. Moreover, these α‐In2Se3 nanosheets exhibit an excellent near‐infrared (NIR) photothermal performance under an 808 nm laser irradiation. NIR photo‐excitation of the α‐In2Se3 nanosheets in the presence of bacteria leads to a significant antibacterial effect, suggesting that these nanosheets have great potential to be photothermal antibacterial agents. Our work on α‐In2Se3 nanosheets presents an available method for exfoliating 2D layered materials, and highlights the potential application in chemical and biological fields of α‐In2Se3 nanosheets.

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The simultaneous quantitation of multiple steroid hormones in real time is of great importance in medical diagnosis. In this study, a portable hormone biosensor based on duplex molecular recognition coupled with a signal-amplified substrate was successfully developed for the simultaneous visualization and quantitation of multiple steroid hormones. Aptamer-functionalized upconversion nanoparticles (UCNPs) with different emission peaks are immobilized on the photonic crystal (PC) substrate as the nanoprobes, leading to the specific and simultaneous assay of multiple steroid hormones. Coupled with the luminescence-enhanced effect of the PC substrate, nanomolar quantification limits of multiple hormones are achieved. This well-designed biosensor is also promising in the quantification of multiple hormones in serum samples. The amplified luminescence signals can be visualized with the naked eye and captured by an unmodified phone camera. This hormone quantitation biosensor exhibits the advantages of multi-detection, visualization, high sensitivity, and selectivity for potential applications in clinical disease diagnosis.