Aerobic granules were cultivated successively in column sequencing batch reactor (SBR) with the glucose as carbon source. The effect of copper (II) on the characteristics of aerobic granules was studied. Results showed that the physical, morphology and extracellular polymeric substances (EPS) of aerobic granules were influenced in different degree under different concentrations of copper (II) in sequencing batch reactor (SBR). The appropriate Cu2+ can be adsorbed by the microorganisms in aerobic granules under the functions of chelation and displacement of heavy metals. Under the Cu2+ concentration of 0.5~3.0mg/L, the stable MLSS and better settleable granules with higher S.G. and lower SVI can been attained in the reactors. The sludge can maintain the granules in stable till the Cu2+ concentration increases to 20.0mg/L. The ratios of EPSC/EPN distribute in 6.60~7.01 steadily under the Cu2+ concentration of 0.5~10.0mg/L.However, excess Cu2+ can destroy the metabolism balance of the microorganisms and the protein synthesization and genetic information transportation are baffled. When the Cu2+ concentration increase to 50mg/L, more filamentous appears in the granules and the compact granules change to in disperse. The ratios of EPSC/EPN evidently rise to 7.59 and 7.46 respectively under high Cu2+ concentration of 20.0mg/L and 50.0mg/L. It suggests that the microorganisms try to excreting more EPS and protecting itself from the environmental change.
Giardia lamblia cysts at low concentrations were detected in water samples using a highly sensitive immunological-PCR (IPCR) method. Magnetic gold particles were precoated with monoclonal anti-Giardia antibodies, and Giardia lamblia cysts ranging from 1 to 100 cysts were diluted in 500 microL of water. A biotinylated detection antibody bound to the G. lamblia cysts was then linked by a streptavidin bridge to biotinylated Giardia-reporter DNA. After extensive washing, reporter DNA was released by denaturation, transferred to PCR tubes, amplified, electrophoresed, and visualized. An optimized immuno-PCR method detected as little as five G. lamblia cysts. To further evaluate the specificity and the clinical application of this IPCR assay for G. lamblia cysts, Entamoeba histolytica and Cryptosporidium parvum were also collected and detected by IPCR. The data demonstrated that this monoclonal antibody-based IPCR method is particularly useful for analysis of environmental water samples in which the densities of G. lamblia cysts is very low, and provides a platform capable of rapid screening of samples from drinking water, wells, rivers, lakes, and recreational swimming pools for trace levels of G. lamblia cysts.
During formation flying, Unmanned Aerial Vehicles (UAV) may need to arrive at target ahead of schedule by hurry path. Given fixed flight high mode, hurry planning method was proposed based on Adaptive Genetic Algorithm (AGA), which makes the new path shorter by locally adjusting the default path. By full considering the risk of UAV flight, the hurry path got by AGA meets the requirements of the risk cost and time amount in advance. Simulation results show that the path gotten by AGA can better meet the requirements of the time amount in advance, and evade the threat area effectively too.
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