Multiple neoplasias including B-cell non-Hodgkin's lymphoma, breast carcinoma, and ovarian carcinoma, have been associated with frequent deletions of the distal region on the long arm of human chromosome 6, suggesting the presence of one or more tumor suppressor gene(s) at this locus. Loss of heterozygosity analysis of breast and ovarian tumors has further restricted the minimal region of loss within 6q27. To further characterize this genomic region for gene content including putative tumor suppressor genes as well as other elements that may contribute to tumorigenesis, a 68940-bp contiguous sequence, encompassing markers D6S193 and D6S297, was generated by random shotgun sequencing of a cosmid, P1, and PAC contig. In addition, exon trapping was performed utilizing a subset of these clones. Sixteen trapped exons, ranging in size from 44 to 399 bp, span this approximately 69-kb region. Many other putative exons have been identified computationally. Further analysis has identified 13 potential promoters and 13 putative polyadenylation sites in the region. Northern analysis identified a transcript mapping within this interval that is expressed in ovarian, breast, and lymphoid-derived tumor cell lines. Consideration of these data, together with the demonstration of several regions of high CpG content, suggests the possibility of several genes at this locus.
After carbonized at different temperatures, agricultural waste-rice-husk, becomes carbonized, which has further been modified at different pH values. Through some static tests, we studied the treatment efficiency for the wastewater--containing mechanical lubricants- by the carbonized rice husk at different amounts of carbonized rice husk, different treatment times and different processing temperatures. The results showed that the optimal carbonization temperature of the rice husk is around 500°C, and the pH modification’s impact was not notable. The optimal conditions of the treatment of wastewater containing 10% mechanical lubricant (volume percentage, made by Shell), were found at the amount of 6g/l carbonized rice husk, with the processing time of 15 min, and the temperature of 15°C. Under that condition, the oil removal rate could reach 88.38% and adsorption capacity of the carbonized rice husk is 22.32ml/g; however, when referring to Mobil (model: 20W-40), the oil removal rate could reach 94.24%, which is considerably superior to other kinds adsorbents. Treatment of oily wastewater by carbonized rice husk is in conformity with the second order kinetic equation, and the related coefficient is 0.9671; besides, the adsorption thermodynamic analysis shows that the adsorption process can be spontaneous at the low temperature, which therefore can be used for the oily waste water treatment.
Activated alumina granules is a primary sorption media . The intention of this experiment is to get out of the difference between the different regenerate medias, caustic soda and alumina sulfate . Experimental result shows the renewal adsorption capacity of activated alumina using caustic soda regenerate is more than using aluminum sulfate .Under the same experiment condition ,aluminum sulfate can regain 13.27mg/g adsorption capacity while using 0.8 per cent caustic soda to regenerate alumina and 5.36mg/g using 4 per cent aluminum sulfate .The intensity of activated alumina granules reduces when we use caustic soda as regenerate media .The mass wastage of activated alumina granules is between 12% and 14% using caustic soda as regenerate media and between 6% and 8%uing aluminum sulfate .It is needed to neutralized by hydrochloric acid or aluminum sulfate after regenerated by caustic soda .Without neutralized, the concentration of treated water is over 1.0mg/l in the beginning.And neutralized with hydrochloric acid or aluminum sulfate, the efficacious run time can achieve 23 hours and 31hours .
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