“Candidatus Liberibacter solanacearum” is a pathogen transmitted by the potato psyllid Bactericera cockerelli (Šulc) (Hemiptera: Triozidae) in a persistent manner. In this study, we investigated the molecular interaction between “Ca. Liberibacter solanacearum” and the potato psyllid at the gut interface. Specifically, we focused on the apoptotic response of potato psyllids to the infection by two “Ca. Liberibacter solanacearum” haplotypes, LsoA and LsoB. To this end, we first quantified and localized “Ca. Liberibacter solanacearum” in the gut of adult psyllids. We then evaluated the existence of an apoptotic response in the insect gut using microscopy analyses to visualize the nuclei and the actin cytoskeleton of the gut cells and DNA fragmentation analyses by agarose gel electrophoresis. We also performed annexin V cell death assays to detect apoptosis. Finally, we annotated apoptosis-related genes from the potato psyllid transcriptome and evaluated their expression in response to “Ca. Liberibacter solanacearum” infection. The results showed no cellular markers of apoptosis despite the large amount of “Ca. Liberibacter solanacearum” present in the psyllid gut. In addition, only three genes potentially involved in apoptosis were regulated in the psyllid gut in response to “Ca. Liberibacter solanacearum”: the apoptosis-inducing factor AIF3 was downregulated in LsoA-infected psyllids, while the inhibitor of apoptosis IAPP5 was downregulated and IAP6 was upregulated in LsoB-infected psyllids. Overall, no evidence of apoptosis was observed in the gut of potato psyllid adults in response to either “Ca. Liberibacter solanacearum” haplotype. This study represents a first step toward understanding the interactions between “Ca. Liberibacter solanacearum” and the potato psyllid, which is crucial to developing approaches to disrupt their transmission.
Vitellogenin-like proteins are members of the large lipid transfer proteins, a family of proteins involved in reproduction, lipid circulation and immune defences. In this study, we identified a new Bactericera cockerelli vitellogenin-like (Vg-like) transcript, and named it BcVg6-like based on its similarity to Acyrthosiphon pisum Vg6. In silico analyses predicted different conserved domains in BcVg6-like compared with the conventional Ba. cockerelli vitellogenin, BcVg1-like, previously described by our research group. Phylogenetic analyses determined that BcVg6-like clustered with Vg-like-B proteins and not the conventional vitellogenins involved in vitellogenesis. Also, the expression analyses showed differences in BcVg6-like transcript expression between 7-day-old males and 3- and 7-day-old females. BcVg6-like was not upregulated after exogenous application of juvenile hormone III, but its relative expression increased significantly in alimentary canals of adult females exposed to tomato plants infected by the bacterial plant pathogen 'Candidatus Liberibacter solanacearum'. Our results suggest that in Ba. cockerelli, both vitellogenin genes may have different functions: BcVg1-like is a conventional vitellogenin that conserved its ancestral function as an egg yolk precursor whereas BcVg6-like might have acquired a function in lipid and/or other molecule transport, and could potentially play a role in immune defence.
SDN is the current hot research topic in the network area. It becomes high-profile because of being considered as the direction of next-generation network. Service deployment is the core content of northbound interface and the focus for researchers in SDN. So far, there are still rare successful SDN application cases, the relevant researches on service deployment are even fewer. Based on the concept of service deployment, we bring up a service deployment framework in SDN, build a service deployment model, and then study the problem of price calculation during the process of service deployment and present some corresponding optimization algorithms.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.