Abstract. In order to understand the effect of mechanical stretch on corneal extracellular matrix remodeling, human keratoconus fibroblasts (HKCFBs) were subjected to cyclic stretch in vitro and the expression of matrix metalloproteinases (MMPs), tissue inhibitor of metalloproteinases (TIMPs), and inflammatory cytokines were evaluated. HKCFBs were seeded into a flexible membrane base and subjected to a cyclic stretch regimen of 10% equibiaxial stretch at a stretching frequency of 1 Hz for 6 h using a Flexcell tension unit. An antibody directed against interleukin-6 (IL-6 Ab) was used to investigate the roles of IL-6 on mechanical stretch mediated regulation of MMP in HKCFBs. Culture supernatants were assayed using an enzyme-linked immunosorbent assay for MMP-1 and -3, TIMP-1 and -2, and IL-6. Total RNA from the cells was extracted, and quantitative polymerase chain reaction was used to determine mRNA for MMP-1 and -3, TIMP-1 and -2, and IL-6. In stretched cells, levels of MMP-1 and -3 demonstrated an increase compared with unstretched cells, but levels of TIMP-1, and -2 revealed a decrease. Mechanical stretch significantly increased the mRNA expression and protein synthesis of IL-6 compared with unstretched cells. IL-6 induced MMP-1 and -3 expression, whereas no significant effects were observed in levels of TIMP-1 and -2 compared with the untreated control groups. Additionally, the IL-6 Ab markedly inhibited the stretch-induced increase in MMP-1 and -3 in culture supernatants in a dose-dependent manner. No significant differences in TIMP-1 and -2 protein levels were detected between stretched cells treated with IL-6 Ab and stretched cells without IL-6 Ab treatment. These results indicate that cyclical mechanical stretch augments IL-6 production and MMP expression, and reduces levels of TIMP in HKCFBs. Thus, it is suggested that IL-6 mediates the stretch-induced MMP expression.
The quantum yield, lifetime, and absorption spectrum of four [Ru(bpy) 2 L] + [where bpy is 2,2Ј-bipyridyl; L is represented by the deprotonated form of 2-(1H-tetrazol-5-yl)pyridine (L1) or 2-(1H-tetrazol-5-yl)pyrazine (L2)], as well as their methylated complexes [Ru(bpy) 2 LMe] 2+ (RuL1Me and RuL2Me) are closely ligand dependent. In this paper, density functional theory (DFT) and time-dependent DFT (TDDFT) were performed to compare the above properties among these complexes. The calculated results reveal that the replacement of pyridine by pyrazine or the attachment of a CH 3 group to the tetrazolate ring greatly increases the π-accepting ability of the ancillary ligands. The stronger π-accepting ability of the ancillary ligands in RuL2 results in stronger interaction with
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