BackgroundDiabetic wounds are a major clinical challenge, because minor skin wounds can lead to chronic, unhealed ulcers and ultimately result in infection, gangrene, or even amputation. Studies on bone marrow derived mesenchymal stem cells (BMSCs) and a series of growth factors have revealed their many benefits for wound healing and regeneration. Platelet-rich plasma (PRP) may improve the environment for BMSC development and differentiation. However, whether combined use of BMSCs and PRP may be more effective for accelerating diabetic ulcer healing remains unclear.ObjectiveWe investigated the efficacy of BMSCs and PRP for the repair of refractory wound healing in a diabetic rat model.MethodsForty-eight rats with diabetes mellitus induced by streptozotocin were divided into four groups: treatment with BMSCs plus PRP, BMSCs alone, PRP alone, phosphate buffered saline. The rate of wound closure was quantified. A histopathological study was conducted regarding wound depth and the skin edge at 7, 14, and 28 days after surgery.ResultsWound healing rates were significantly higher in the BMSC plus PRP group than in the other groups. The immunohistochemistry results showed that the expression of platelet/endothelial cell adhesion molecule 1, proliferating cell nuclear antigen, and transforming growth factor-β1 increased significantly in the BMSC plus PRP group compared to the other treatment groups. On day 7, CD68 expression increased significantly in the wounds of the BMSC plus PRP group, but decreased markedly at day 14 compared to the controls.ConclusionThe combination of BMSCs and PRP aids diabetic wound repair and regeneration.

Because of the communication distance, the more nodes, the requirements of high real-time, LED lighting control system for railway vehicle carriage chooses Controller Area Network (CAN) as the communication mode to achieve centralized control in all carriages. Illuminance gathered from multi-sensors which were installed on the surface of the carriages was feed back to the Industrial Personal Computer (IPC). And then, through the incremental PID control algorithm, the IPC achieve the brightness of the lighting system intelligent control for vehicle consulting brightness intensity which according to the external environment. Experiment shows that this method is practical and effective.

For the use of CNC machine tools is more and more extensive, its fault diagnosis research is becoming more and more important. Failure forms are diversified, and fault reasons are very complicated. Once bearing that is an important transmission part is in fault, the machining efficiency will decrease and affect process accuracy, even make the machine work abnormally. This paper presents a deep analysis of bearing failure mechanism using the wavelet analysis method identify its failure, specially the motor and transmission machine bearing, which provides reliable basis for fault diagnosis and forecast.

According to the ineffective denitrification and the initial controlling technique flaws for low-carbon source in reversed A2/O process, and full scale experimental studies were conducted to strengthen denitrification at normal and low temperature days in 2008~2010, to ensure the best conditions of biological denitrification and promote the stable and efficient operation of the system. The results showed that in normal temperature seasons of 2008, after shortening the HRT of the primary sedimentation tank to 1/3 of design value, improving the MLSS to 4500mg/L, setting the first section of the aerobic zone as the denitrification transition section and improving the reflux ratio, available carbon source was increased by 15%, the effluent NH3-N was 2.5mg / L and the removal rate was 90%, the effluent TN was 17 mg/L and the removal rate was increased to 54% and unit power consumption was reduced by 15% to 0.22Kw•h/m3.At low temperature seasons of 2008~2009,by improving the MLSS to 6000mg/L, extending the sludge age to enrich a large number of nitrification and denitrification bacteria, controlling DO at about 1.2mg / L in the aerobic zone and so on, the effluent NH3-N was 3 mg / L and the removal rate was 88%, the effluent TN was 15.5 mg/L and the removal rate was 62%. In low temperature of 2009 ~2010, besides the same measures as before, adding a blender in the first section of the aerobic zone to provide a better anoxic environment for denitrification and to improve nitrogen removal, the effluent NH3-N was 3 mg / L and the removal rate was 87%, the effluent TN was 13.5 mg/L and the removal rate was 66%.