Daunorubicin (DNR), an antibiotic-antimetabolite, was used as an adjunct to standardized partialthickness filtration surgery in 21 rabbits to determine its effects on that surgery. Postoperatively, one of the eyes of each rabbit received subconjunctival injections of DNR 25 µg or 50 µg, while the fellow eyes received only diluent, daily for 2 to 14 days. Bleb survival, fistula patency, the thickness of the subconjunctival connective tissue, and postoperative complications were investigated. The filtration blebs lasted significantly longer (P < .005), the rate of fistula closure was significantly lower (P < .005), and the subepithelial connective tissue was much looser and thicker in the experimental eyes than in the control eyes. Corneal toxicity occurred more frequently in the eyes treated with the higher-dose DNR than in those treated with the lower dose and in the control eyes.
GH425 gene comes from the Grimmia pilifera drought stress of cDNA library.In this experiment,we have got the full sequence of GH425NO.1 by E-cloning which using GH425 as gene probe,in Physcomitrella patens DNA Datebase.Through using ORFfinder to find out the longest ORF and design primer for it,then, validated the Physcomitrella patens by PT-PCR,and we have obtained corresponding band and proved that the result of silicon cloning is correct and the fragment is contained in Grimmia pilifera P.Beauv.Now,we know the sequence encodes Eukaryotic initiation factor 4E by Blastx,and analysis it with Bioinformatics.
Blood samples were collected from unrelated individuals of Chinese Han ethnic group in Chengdu of China. DNAwas extracted using Chelex method (1). PCR amplification conditions can be accessed at http://www.legalmed.org/dna/DXS6809.htm (2). The volume of PCR reaction for each locus was 37.5 µL. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by sliver staining (2,3). Data were analyzed using POWERSTATS program (4).
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