Excessive generation of reactive oxygen species (ROS) in cancer cells is associated with cancer development, but the underlying mechanisms and therapeutic significance remain elusive. In this study, we reported that levels of ROS and p22(phox) expression are greatly increased in human prostate cancer tissues, and knockdown of p22(phox) by specific small interfering RNA (siRNA) decreased ROS levels in prostate cancer cells. We also showed that stable downregulation of p22(phox) in prostate cancer cells inhibited cell proliferation and colony formation, which was mediated by AKT and extracellular signal-regulated kinase (ERK)1/2 signaling pathways and their downstream molecules hypoxia-inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF). The NADPH oxidase subunit NOX1 was also elevated in prostate cancer cells, and was involved in activation of AKT/ERK/HIF-1/VEGF pathway and regulation of cell proliferation. Knockdown of p22(phox) resulted in inhibition of tumor angiogenesis and tumor growth in nude mice. These findings reveal a new function of p22(phox) in tumor angiogenesis and tumor growth, and suggest that p22(phox) is a potential novel target for prostate cancer treatment.
Abstract. The regularity of radon exhalation rate in the over-broken granite tunnel is susceptible to weather conditions and ventilation styles. Based on the calculation model of radon exhalation in tunnel, some experiments have been carried out to analyze the variations of radon exhalation in cases of natural ventilation, blowing ventilation and exhaust ventilation separately. The results show that there is a linear relation between the radon exhalation and the natural ventilation quantity, and also between the radon exhalation and the ambient temperature; the radon exhalation in the case of exhaust ventilation is 63% higher than that in the blowing case under the condition of the same ventilation quantity and ambient temperature. Therefore, it is suggested that operation in the tunnel in high temperature be avoided in summer, and the blowing ventilation be adopted as an effective way for ventilation.
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