Background
Previous in vitro studies demonstrated that suppression of microRNAs might protect cardiomyocytes and neurons against oxygen–glucose deprivation and reoxygenation (OGD/R)-induced cell apoptosis. However, whether the protective effect of miR-153-inhibition on cardiomyocytes can be observed in the animal model is unknown. We aimed to address this question using a rat model of ischemia–reperfusion (I/R).
Methods
Rats were received the intramyocardial injection of saline or adenovirus-carrying target or control gene, and the rats were subjected to ischemia/reperfusion (I/R) treatment. The effects of miR-153 on I/R-induced inflammatory response and oxidative stress in the rat model were assessed using various assays.
Results
We found that suppression of miR-153 decreased cleaved caspase-3 and Bcl-2-associated X (Bax) expression, and increased B cell lymphoma 2 (Bcl-2) expression. We further confirmed that Nuclear transcription factor erythroid 2-like 2 (Nrf2) is a functional target of miR-153, and Nrf2/Heme oxygenase-1 (HO-1) signaling was involved in miR-153-regulated I/R-induced cardiomyocytes apoptosis. Inhibition of miR-153 reduced I/R-induced inflammatory response and oxidative stress in rat myocardium.
Conclusion
Suppression of miR-153 exerts a cardioprotective effect against I/R-induced injury through the regulation of Nrf2/HO-1 signaling, suggesting that targeting miR-153, Nrf2, or both may serve as promising therapeutic targets for the alleviation of I/R-induced injury.
With the development of web service, more and more attention has been drawn to web service in recent years. However, the damage caused by service security problems is becoming more and more serious nowadays. This paper develops a web service security analysis tool, WSSecTool, which is based on program slicing. This tool includes three modules namely slicing module, safety publishing module and testing module. Slicing module analyzes source codes of web service to generate method dependence graph (MDG). After MDG is generated, the tool slices MDG to detect the unsafe methods and the spread of them. Safety publishing module helps to hide unsafe methods to make them invisible to outer users after publishing. Testing module can test the services published by this tool to validate the correctness and feasibility of our analysis method. The experiments show that our tool is effective and practical.
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