We aimed to investigate the regulatory mechanism of lentivirus-mediated overexpression of cystic fibrosis transmembrane conductance regulator (CFTR) in oxidative stress injury and inflammatory response in the lung tissue of mouse model of chronic obstructive pulmonary disease (COPD). COPD mouse model induced by cigarette smoke was established and normal mice were used as control. The mice were assigned into a normal group (control), a model group (untreated), an oe-CFTR group (injection of lentivirus overexpressing CFTR), and an oe-NC group (negative control, injection of lentivirus expressing irrelevant sequences). Compared with the oe-NC group, the oe-CFTR group had higher CFTR expression and a better recovery of pulmonary function. CFTR overexpression could inhibit the pulmonary endothelial cell apoptosis, reduce the levels of glutathione (GSH), reactive oxygen species (ROS), and malondialdehyde (MDA) and increase the values of superoxide dismutase (SOD), GSH peroxidase (GSH-Px), and total antioxidant capacity (T-AOC). The overexpression also led to reductions in the white blood cell (WBC) count in alveolus pulmonis, the concentrations of C-reactive protein (CRP), interleukin (IL)-6, and tumor necrosis factor-α, and the protein expressions of NF-κB p65, ERK, JNK, p-EPK, and p-JNK related to MAPK/NF-κB p65 signaling pathway. In conclusion, CFTR overexpression can protect lung tissues from injuries caused by oxidative stress and inflammatory response in COPD mouse model. The mechanism behind this may be related to the suppression of MAPK/NF-κB p65 signaling pathway.
The aim of the present study was to identify the potential target biomarkers associated with burn sepsis using microarray. GSE1781 was downloaded from Gene Expression Omnibus and included a collective of three biological replicates for each of the three conditions: Sham‑Sham, Sham‑cecal ligation and puncture (CLP) and Burn‑CLP. Subsequently, limma was applied to screen the differentially expressed genes (DEGs). Additionally, functional annotations were predicted by pathway enrichment. Furthermore, the transcription factors were screened according to the transcriptional regulation from patterns to profiles database. Furthermore, the interaction associations of the proteins were obtained from the STRING database and the protein‑protein interaction (PPI) network was constructed using Cytoscape. Finally, the gene co‑expression analysis was conducted using CoExpress. In total, compared with Sham‑Sham, a total of 476 DEGs and 682 DEGs were obtained in Sham‑CLP and Burn‑CLP, respectively. Additionally, 230 DEGs were screened in Burn‑CLP compared with Sham‑CLP. Acadm, Ehhadh and Angptl4 were significantly enriched in the PPAR signaling pathway. Additionally, Gsta3, Gstm2 and Gstt1 in Burn‑CLP were significantly enriched in glutathione metabolism. In the PPI network, the transcription factor Ppargc1a interacted with Angptl4, while Acadm interacted with Ehhadh. The gene co‑expression analysis showed that Ehhadh could be co‑expressed with Aqp8. In conclusion, Acadm, Ehhadh, Aqp8, Gsta3, Gstm2, Gstt1, Ppargc1a and Angptl4 may be potential target genes for the treatment of burn sepsis.
Based on the gene expression profile of patients with ventilator-associated pneumonia (VAP) and patients not affected by the disease, the present study aimed to enhance the current understanding of VAP development using bioinformatics methods. The expression profile GSE30385 was downloaded from the Gene Expression Omnibus database. The Linear Models for Microarray Data package in R language was used to screen and identify differentially expressed genes (DEGs), which were grouped as up- and down-regulated genes. The up- and downregulated genes were functionally enriched using the Database for Annotation, Visualization and Integrated Discovery system and then annotated according to TRANSFAC, Tumor Suppressor Gene and Tumor Associated Gene databases. Subsequently, the protein-protein interaction (PPI) network was constructed, followed by module analysis using CFinder software. A total of 69 DEGs, including 33 up- and 36 downregulated genes were screened out in patients with VAP. Upregulated genes were mainly enriched in functions and pathways associated with the immune response (including the genes ELANE and LTF) and the mitogen-activated protein kinase (MAPK) signaling pathway (including MAPK14). The PPI network comprised 64 PPI pairs and 44 nodes. The top two modules were enriched in different pathways, including the MAPK signaling pathway. Genes including ELANE, LTF and MAPK14 may have important roles in the development of VAP via altering the immune response and the MAPK signaling pathway.
Background Tracheostomy is very common in patients with severe traumatic brain injury (TBI), long‐term nursing care are needed for those patients. We aimed to evaluate the effects of hospital‐community‐home (HCH) nursing in those patients. Methods This study was a before‐after study design. Patients were divided into control groups (traditional nursing care) and HCH group(HCH nursing care). Tracheostomy patients with severe TBI needing long‐term care were included. All patients underwent a two‐month long follow‐up. Glasgow coma score (GCS), Karnofsky, Self‐Anxiety Scale (SAS) from caregiver and Barthel assessment at the discharge and two months after discharge were evaluated. The tracheostomy‐related complications were recorded and compared. Results A total of 60 patients were included. There were no significant differences between the two groups in the GCS, Karnofsky, SAS from caregiver and Barthel index at discharge((all P > .05); the GCS, Karnofsky and Barthel index were all significantly increased after two‐month follow‐up for the two groups (all P < .05), and the GCS, Karnofsky and Barthel index at two‐month follow‐up in HCH group were significantly higher than that of the control group(all P < .05), but the SAS from caregiver at two‐month follow‐up in HCH group was significantly less than that of the control group(P = .009). The incidence of block of artificial tracheal cannula and readmission in HCH group were significant less than that of control group (all P < .05). Conclusion HCH nursing care is feasible in tracheostomy patients with severe TBI, future studies are needed to further evaluate the role of HCH nursing care.
IntroductionEnteral nutrition support is very important to improve the prognosis of patients with traumatic brain injury (TBI). We aimed to assess the role of duodenal versus gastric feeding in TBI patients, to provide insights into the clinical practice and nursing care.Material and methodsWe searched PubMed and other databases for RCTs on the role of duodenal versus gastric feeding in TBI patients up to Dec 15, 2021. Cochrane Collaborations risk of bias tool was used to assess the methodological quality and risk of bias of included studies. The RevMan 5.3 software was used for data analysis, risk rate (RR) or mean differences (MDs) with 95% confidence interval (CI) were calculated, and publication bias were evaluated by funnel plots.ResultsA total of 16 RCTs were included in this meta-analysis. Synthesized outcomes indicated that compared with gastric feeding, duodenal feeding is beneficial to reduce the incidence of pneumonia[RR =0.46, 95% CI (0.38, 0.57)], aspiration[RR =0.30, 95% CI (0.14, 0.63)], reflux esophagitis[RR =0.25, 95% CI (0.17, 0.38)], diarrhea [RR =0.58, 95% CI (0.44, 0.77)], abdominal distension[RR =0.41, 95% CI (0.25, 0.68)], no significant difference in the mortality[RR =0.85, 95% CI (0.50, 1.47), P=0.57] was found. Egger regression test indicated that there were no publication biases in the synthesized outcomes (all p>0.05).ConclusionsDuodenal feeding may be superior to gastric feeding in the treatment and nursing care of TBI patients with less complications. Future studies with larger sample size and rigorous design are needed to further elucidate the effects and safety of duodenal versus gastric feeding.
Background Tracheostomy is very common in patients with severe traumatic brain injury (TBI), and long-term nursing care are needed for those patients. We aimed to evaluate the effects of hospital-community-home (HCH) nursing in those patients. Methods Tracheostomy patients with severe TBI were included. Glasgow coma score (GCS), Karnofsky, Self-Anxiety Scale (SAS) and Barthel assessment at the discharge and two months after discharge were evaluated. Results A total of 60 patients were included. There weren’t significant differences between two groups in the GCS, Karnofsky, SAS and Barthel index at discharge((all p > 0.05), the GCS, Karnofsky and Barthel index was all significantly increased after two months follow-up for two groups (all p < 0.05), and the GCS, Karnofsky and Barthel index at two months follow-up in HCH group was significantly higher than that of control group(all p < 0.05), but the SAS at two months follow-up in HCH group was significantly less than that of control group(p = 0.009). The incidence of block of artificial tracheal cannula and readmission in HCH group were significant less than that of control group (all p < 0.05). Conclusion HCH nursing care is feasible in tracheostomy patients with severe TBI, future studies are needed to further evaluate the role of HCH nursing care.
Background: Tracheostomy is very common in patients with severe traumatic brain injury (TBI), and long-term nursing care are needed for those patients. We aimed to evaluate the effects of hospital-community-home (HCH) nursing in those patients. Methods: Tracheostomy patients with severe TBI needing long-term care were included. All patients underwent two months long follow-up. Glasgow coma score (GCS), Karnofsky, Self-Anxiety Scale (SAS) (SAS) and Barthel assessment at the discharge and two months after discharge were evaluated. The tracheostomy related complications were recorded and compared.Results: A total of 60 patients were included. There weren’t significant differences between two groups in the GCS, Karnofsky, SAS and Barthel index at discharge((all p>0.05), the GCS, Karnofsky and Barthel index was all significantly increased after two months follow-up for two groups (all p<0.05), and the GCS, Karnofsky and Barthel index at two months follow-up in HCH group was significantly higher than that of control group(all p<0.05), but the SAS at two months follow-up in HCH group was significantly less than that of control group(p=0.009). The incidence of block of artificial tracheal cannula and readmission in HCH group were significant less than that of control group (all p<0.05).Conclusion: HCH nursing care is feasible in tracheostomy patients with severe TBI, future studies are needed to further evaluate the role of HCH nursing care.
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