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Abstract. E-learning is becoming more and more popular, which is a kind of learning mode based on network, with the dramatic development of computer and network technology. Although e-learning system is bringing about an education revolution, the lack of emotion and interactivity always persecute students and researchers. In this paper, a system architecture based on e-learning is proposed, which focused on interaction. In order to prompt students' experience of learning, we analyze the relationship of students' emotion and teaching content based on Kansei Engineering. A novel approach is proposed to evaluate students' emotion to teaching content, which can improve students' learning efficiency.

<p>Supplementary Table S1: Clinicopathological features of osteosarcoma patients Supplementary Table S2: Primer sequences used for Quantitative PCR Supplementary Table S3: Primers used for ChIP experiments Supplementary Table S4: Tumor forming ability following subcutaneous injections</p>

<p>Figure S1. Notch promotes osteosarcoma progression Figure S2. Notch pathway inhibits Eph forward signaling</p>

<div>Abstract<p>Despite significant advancements in the diagnosis and treatment of osteosarcoma, the molecular mechanisms underpinning disease progression remain unclear. This work presents strong clinical and experimental evidence demonstrating that Notch signaling contributes to osteosarcoma progression. First, using a cohort of 12 patients, Notch genes were upregulated in tumors compared with adjacent normal tissue, and high tumor expression of Notch1 intercellular domain (NICD1) and the Notch target gene <i>Hes1</i> correlated with poor chemotherapy response. Data mining of publicly available datasets confirmed that expression of Notch pathway genes is related to poor prognosis in osteosarcoma. On the basis of <i>in vitro</i> analysis, Notch signaling promoted osteosarcoma proliferation, enhanced chemoresistance, facilitated both migration and invasion, and upregulated stem cell–like characteristics. Xenograft models demonstrated that Notch signaling promotes primary tumor growth and pulmonary metastasis, and Notch inhibition is effective in reducing tumor size and preventing metastasis. Mechanistically, activated Notch signaling induces the expression of ephrinB1 and enhances the tumor-promoting ephrin reverse signaling. Overall, these findings provide functional evidence for Notch pathway genes as candidate biomarkers to predict prognosis in patients with osteosarcoma, and suggest a mechanistic rationale for the use of Notch inhibitors to treat osteosarcoma.</p>Implications:<p>The study provides preclinical evidence for Notch pathway as a molecular marker to predict osteosarcoma prognosis and as a therapeutic target against osteosarcoma. In addition, we identified a novel mechanism that ephrin reverse signaling acts as a key mediator of Notch pathway.</p></div>

<p>Figure S1. Notch promotes osteosarcoma progression Figure S2. Notch pathway inhibits Eph forward signaling</p>