Paramecium bursaria Chlorella virus type 1 (PBCV-1) is a very large, icosahedral virus containing an internal membrane enclosed within a glycoprotein coat consisting of pseudohexagonal arrays of trimeric capsomers. Each capsomer is composed of three molecules of the major capsid protein, Vp54, the 2.0-Å resolution structure of which is reported here. Four N-linked and two O-linked glycosylation sites were identified. The N-linked sites are associated with nonstandard amino acid motifs as a result of glycosylation by virus-encoded enzymes. Each monomer of the trimeric structure consists of two eight-stranded, antiparallel -barrel, ''jelly-roll'' domains related by a pseudo-sixfold rotation. The fold of the monomer and the pseudo-sixfold symmetry of the capsomer resembles that of the major coat proteins in the double-stranded DNA bacteriophage PRD1 and the double-stranded DNA human adenoviruses, as well as the viral proteins VP2-VP3 of picornaviruses. The structural similarities among these diverse groups of viruses, whose hosts include bacteria, unicellular eukaryotes, plants, and mammals, make it probable that their capsid proteins have evolved from a common ancestor that had already acquired a pseudo-sixfold organization. The trimeric capsid protein structure was used to produce a quasi-atomic model of the 1,900-Å diameter PBCV-1 outer shell, based on fitting of the Vp54 crystal structure into a three-dimensional cryoelectron microscopy image reconstruction of the virus.
Paramecium bursaria Chlorella virus type 1 (PBCV-1) is a very large, icosahedral virus containing an internal membrane enclosed within a glycoprotein coat consisting of pseudohexagonal arrays of trimeric capsomers. Each capsomer is composed of three molecules of the major capsid protein, Vp54, the 2.0-Å resolution structure of which is reported here. Four N-linked and two O-linked glycosylation sites were identified. The N-linked sites are associated with nonstandard amino acid motifs as a result of glycosylation by virus-encoded enzymes. Each monomer of the trimeric structure consists of two eight-stranded, antiparallel -barrel, ''jelly-roll'' domains related by a pseudo-sixfold rotation. The fold of the monomer and the pseudo-sixfold symmetry of the capsomer resembles that of the major coat proteins in the double-stranded DNA bacteriophage PRD1 and the double-stranded DNA human adenoviruses, as well as the viral proteins VP2-VP3 of picornaviruses. The structural similarities among these diverse groups of viruses, whose hosts include bacteria, unicellular eukaryotes, plants, and mammals, make it probable that their capsid proteins have evolved from a common ancestor that had already acquired a pseudo-sixfold organization. The trimeric capsid protein structure was used to produce a quasi-atomic model of the 1,900-Å diameter PBCV-1 outer shell, based on fitting of the Vp54 crystal structure into a three-dimensional cryoelectron microscopy image reconstruction of the virus.
Chilo iridescent virus (CIV) (genus Iridovirus, family Iridoviridae) infects the rice stem borer insect [1]. The CIV virion (~1x10 9 Da) encapsidates a 209 kbp dsDNA genome that is surrounded by a lipid membrane (~9% of total virion weight) [2]. More than 30 polypeptides can be differentiated with 2D gel electrophoresis [2]. The major capsid protein (MCP), P50 (467 amino acids, 51.4 kDa), and that of PBCV-1, Vp54, share ~22% sequence identity [3][4][5]. The published 3D image reconstruction of CIV at 26Å resolution revealed that the protein shell (maximum diameter of 1850Å) consists of 12 pentavalent (pentamer) and 1460 hexavalent (trimer) capsomers, arranged with T=147 (h = 7, k = 7) icosahedral, quasi-equivalent symmetry [6]. The capsomers are organized into 12 pentasymmetrons and 20 trisymmetrons. Each pentasymmetron consists of one pentamer plus 30 trimers, whereas each trisymmetron consists of 55 trimers [6]. In this study, we have improved the resolution of the CIV image reconstruction to 13Å.Purified CIV samples were prepared for cryo-electron microscopy as described [6]. Images were recorded in an FEI/Philips CM300 FEG electron microscope at 33,000x nominal magnification with defocus levels ranging from 0.8 to 3.0 m. A total of 6925 CIV particle images were extracted from 180 micrographs (4.24Å pixel step size), and 1800 of these images were used to compute a 3D reconstruction to a resolution limit of 13Å. Model-based procedures were used to determine and refine the particle origins and orientations with the previously published CIV reconstruction serving as the initial model [6,7]. The program SITUS was used to dock an atomic model of the PBCV-1 Vp54 trimer into each of the 25 trimers in the asymmetric unit of CIV [8].The latest 3D image reconstruction of CIV reveals an enhanced view of the capsid shell, the protruding fibers, and the underlying bilayer membrane (Fig. 1A-D). Each trimeric capsomers exhibits a hexagonal profile and a central fiber that extends along the trimer axis. The fibers have both rigid (proximal part where density is highest) and flexible (distal part where density is weaker and smeared out) components. The bilayer membrane, which exhibits the characteristic railway track appearance, follows the general contour of the capsid shell. Some additional and slightly weaker densities occur between the capsid shell and the bilayer membrane. These densities may be responsible for tethering the lipid membrane to the outer capsid and thereby help maintain its icosahedral shape. The atomic model of Vp54 nicely fits within the reconstructed density of all CIV trimers. This result is consistent with the hypothesis that the MCPs of many large dsDNA viruses have closely similar tertiary structures despite only sharing weak sequence identity, and that these proteins may have evolved from a common ancestor [9].
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