The black rockfish (Sebastes schlegelii) is a teleost in which eggs are fertilized internally and retained in the maternal reproductive system, where they undergo development until live birth (viviparity). In the present study, we report a chromosome‐level black rockfish genome assembly. High‐throughput transcriptome analysis (RNA‐seq and ATAC‐seq) coupled with in situ hybridization (ISH) and immunofluorescence reveal several candidate genes for maternal preparation, sperm storage and release, and hatching. We propose that zona pellucida (ZP) proteins retain sperm at the oocyte envelope, while genes in two distinct astacin metalloproteinase subfamilies serve to release sperm from the ZP and free the embryo from chorion at prehatching stage. We present a model of black rockfish reproduction, and propose that the rockfish ovarian wall has a similar function to the uterus of mammals. Together, these genomic data reveal unprecedented insights into the evolution of an unusual teleost life history strategy, and provide a sound foundation for studying viviparity in nonmammalian vertebrates and an invaluable resource for rockfish ecological and evolutionary research.
Abstract:Methane emissions from hydroelectric reservoirs can comprise a considerable portion of anthropogenic methane. However, lack of data on CH 4 emissions in different geographical regions and high spatial-temporal variability in the emission rates of reservoirs has led to uncertainties regarding regional emission estimates of CH 4 . In the subtropical plateau climate region, we used the Ertan hydroelectric reservoir as a study area. The CH 4 flux at the air-water interface was assessed by floating chambers and factors influencing emissions, including the distance from the dam, water depth, seasonal variation in wet and dry season, air-water temperature gradient and wind speed, and was also studied through a year-long systematic sampling and monitoring experiment. The results showed that the surface of the reservoir was a source of CH 4 during the sampling period and the annual average CH 4 flux was 2Ð80 š 1Ð52 mg m 2 d 1 . CH 4 flux (and its variation) was higher in the shallow water areas than in the deep-water areas. CH 4 flux near the dam was significantly higher than that of other locations farther from the dam in the dry season. The seasonal variations of CH 4 emission in wet and dry seasons were minor and significant diurnal variations were observed in wet and dry seasons. Exponential relationships between the CH 4 flux and air-water temperature gradient were found. Air-water temperature gradient was an important factor influencing diurnal variations of CH 4 flux in the Ertan hydroelectric reservoir. These results indicate that systematic sampling is needed to better estimate CH 4 flux through coverage of the spatial variation of different water depths, measuring-point distance from the dam, seasonal variation in wet and dry seasons and changes in climate factors (such as air-water temperature gradient). Our results also provide a fundamental parameter for CH 4 emission estimation of global reservoirs.
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