Anthocyanin biosynthesis in various plants is affected by environmental conditions and controlled by the transcription level of the corresponding genes. In pears (Pyrus communis cv. ‘Wujiuxiang’), anthocyanin biosynthesis is significantly induced during low temperature storage compared with that at room temperature. We further examined the transcriptional levels of anthocyanin biosynthetic genes in ‘Wujiuxiang’ pears during developmental ripening and temperature-induced storage. The expression of genes that encode flavanone 3-hydroxylase, dihydroflavonol 4-reductase, anthocyanidin synthase, UDP-glucose: flavonoid 3-O-glucosyltransferase, and R2R3 MYB transcription factor (PcMYB10) was strongly positively correlated with anthocyanin accumulation in ‘Wujiuxiang’ pears in response to both developmental and cold-temperature induction. Hierarchical clustering analysis revealed the expression patterns of the set of target genes, of which PcMYB10 and most anthocyanin biosynthetic genes were related to the same cluster. The present work may help explore the molecular mechanism that regulates anthocyanin biosynthesis and its response to abiotic stress at the transcriptional level in plants.
In this study, we investigated antibacterial activity of zinc oxide (ZnO) nanoparticles coated on polyvinyl chloride (PVC) films against Escherichia. coli both in vitro and in actual test. Results showed that the nano-ZnO coated films displayed excellent inhibition effects on the growth of E. coli and the nano-ZnO particular was contributed to the bactericidal ability. The more amounts of the ZnO particulars the film coated, the greater inhibitory effect it exhibited. The disinfection efficiency with ZnO film is relatively constant at pH values in the range of 4.5 to 8.0. In the actual test, the number of E. coli cells from cut apple stored in a ZnO-coated bag in the dark decreased from 8.72 to 6.3 log CFU/ml after 1 day, while that of an same bag irradiated with light decreased from 8.72 to 3.5 log CFU/ml after 2 days of storage. The results reveal that nano-ZnO coated film has a good promise to make antimicrobial packaging again E. coli and reduce the risks of microbial growth on fresh-cut produce.
The effects of a novel nano-ZnO coated PVC film on physicochemical quality and microbiological changes of fresh-cut ‘Fuji’ apple were evaluated. Fruit decay were efficiently reduced in nano-packing samples. The content of ascorbic acid and total phenolic dropped to 0.2 g•kg-1, 1.2 g•kg-1 in nano-packing samples and 0.02 g•kg-1, 0.6 g•kg-1 in control on day 12. In addition, the nano-ZnO coated PVC film not only inhibited the growth of total aerobic psychrophilic microorganisms, the count of which did not exceed 5 log cfu g−1 FW, but also suppressed the increasing of yeast and mould, the maximum growth of which reached only 3.6 log cfu g−1 in the nano-packing but 6 log cfu g−1 in control.
This paper explains and demonstrates the effects of beeswax on functional and structural properties of soy protein isolate films, containing different glycerol. The results showed that percentage elongation at break, water vapor permeability, and transparency of soy protein isolate films decreased when the beeswax content increased, but tensile strength and oxygen permeability increased. The higher the glycerol content, the higher the film water vapor permeability, oxygen permeability, and transparency. The results of differential scanning calorimetry and Fourier transform infrared spectroscopy suggested that beeswax cross-linked with soy protein isolate molecules via connecting with glycerol, composed the film matrix.
In this investigation, antibacterial property of microencapsulated cinnamon oil was investigated. Microencapsulated cinnamon oil was prepared by simple coacervation. Microencapsulated cinnamon oil exhibited good antimicrobial activities.Moreover, the effect of microencapsulated cinnamon oil on the quality of cherry tomato was investigated. Result showed microencapsulated cinnamon oil could reduced fruit decay and keep the quality of cherry tomato.
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