Summary. -We investigated the effect of growth phase of suspension culture of insect Sf9 cells on cell cycle phase distribution, cell viability and Autographa californica multiple nucleopolyhedrovirus (AcMNPV) production. The cell culture showed a maximum cell viability and potential to replicate the virus at the peak of G 1 phase cells in the culture, while the minimum cell viability coincided with the peak of G 2 /M phase cells. These results indicate that the G 1 phase plays a substantial role in the ability of cells to replicate the baculovirus and may help to develop a baculovirus infection dynamics model and control the expression of foreign genes.Keywords: cell cycle; baculovirus; viability; flow cytometry; infection E-mail: yhz@mail.wit.edu.cn; phone: +86-13871114386. Abbreviations: AcMNPV = Autographa californica multiple nucleopolyhedrovirus; BEVS = baculovirus expression vector system; FCM = flow cytometry; NOV = non-occluded virus; OV = occluded virus; TOI = time of infection
Abstract. Recently, smoggy weather has become a daily in large part of China because of rapidly economic growth and accelerative urbanization. Stressed on the smoggy situation and economic growth, the green and environment-friendly technology is necessary to reduce or eliminate the smog and promote the sustainable development of economy. Previous studies had confirmed that nitrogen oxides ( NOx ) is one of crucial factors which forms smog. Microorganisms have the advantages of quickly growth and reproduction and metabolic diversity which can collaboratively Metabolize various NOx. This study will design a kind of bacteria & algae cultivation system which can metabolize collaboratively nitrogen oxides in air and intervene in the local nitrogen cycle. Furthermore, the nitrogen oxides can be transformed into nitrogen gas or assembled in protein in microorganism cell by regulating the microorganism types and quantities and metabolic pathways in the system. Finally, the smog will be alleviated or eliminated because of reduction of nitrogen oxides emission. This study will produce the green developmental methodology.
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