The objective of this study was to determine the dry matter (DM) and crude protein (CP) degradation parameters of several commercially available concentrate feed ingredients used in Shaanbei White Cashmere Goat (SWCG). Three 4-year-old female SWCGs fitted with permanent rumen fistula were fed with corn straw and concentrate feed. Tested energy and protein feed ingredients were corn, wheat bran (WB), corn germ meal, soybean meal (SBM), rape seed meal (RSM), and distillers dried grains. The feed samples were placed in a nylon bag and incubated in the rumen for 0, 2, 6, 12, 24, 36, and 48 h to measure the ruminal disappearance of DM and CP. The exponential model was used to calculate the degradation kinetics. There were significant differences between sources in terms of DM and CP disappearance and degradability. In terms of energy feed ingredients, the potential degradability of DM and CP of corn was significantly higher than those of the other energy feed ingredients, whereas the effective degradability (ED) of CP of WB at three outflow rates was significantly higher than those of the others. In protein feed ingredients, SBM had the highest potential degradability of DM and CP. Furthermore, the ED of DM of SBM at three outflow rates was significantly higher than those of the other protein feed ingredients, whereas the ED of CP of RSM at three outflow rates was significantly higher than those of others. Those feed ingredients with low degradability can increase the bypass nutrients, which will be digested in the small intestine. The degradation parameters obtained in this experiment using SWCG would be useful in improving the accuracy of formulation of cashmere goat diet in Northwest of China. ARTICLE HISTORY
In the present study, the second nuclear internal transcribed spacer (ITS-2) rDNA of Schistosoma japonicum isolates in mainland China was amplified, sequenced, and assessed for inferring the intra- and inter-species phylogenetic relationships of trematodes in the order Strigeata. The fragment containing ITS-2 rDNA was obtained from 24 S. japonicum isolates from eight epidemic provinces in mainland China. The length polymorphisms were observed among these ITS-2 rDNA sequences, ranging from 343 to 346 bp, and the intra- and inter-population variations in ITS-2 sequence were 0.0-2.1% among S. japonicum isolates in China. Phylogenetic analyses using the maximum parsimony and maximum likelihood methods revealed that the ITS-2 rDNA sequence is not a suitable marker for studying inter- and intra-population variation in S. japonicum. However, phylogenetic analysis of trematodes in the order Strigeata indicated that the ITS-2 rDNA sequence provides an effective molecular marker for studying inter-species phylogenetic relationships among trematodes in this order.
It has been shown that the oxidized low density lipoprotein receptor 1 (OLR1) gene plays an important role in the degradation of oxidized low density lipoprotein. Previous studies found a SNP in the 3'-untranslated region (3'-UTR) of the OLR1 gene associated with milk production traits in different dairy cattle populations and with loin eye area and marbling depth in beef cattle. MicroRNAs can regulate gene expression by binding the 3'-UTR of target genes to degrade or to repress the translation of target genes. Bioinformatics have shown that there is a binding site of bta-miR-370 in the 3'-UTR of the OLR1 gene, and a previous luciferase reporter assay system showed that the A/C mutation occurring in the 3'-UTR of this gene caused the binding sites of bta-miR-370 to disappear in HEK293 cells. To further validate whether OLR1 was the target gene of bta-miR-370, the over-expression and interference expression of bta-miR-370 were determined by transfecting bta-miR-370 mimics and inhibitor supplementations into bovine adipocyte. The qRT-PCR result showed that the relative expression of OLR1 gene significantly decreased in the mimics group compared to the control, whereas the expression level in inhibitor group was higher than its control group. The above results were further verified by a Western blot at the protein level. In addition, lipid formation analysis of bovine adipocytes was performed via oil red O staining, and we found that cytoplasm lipid droplets in the inhibitor group showed a tendency to increase compared to the control group, whereas in the mimics group, we observed an obvious decrease of cytoplasm lipid droplets compared to the control and inhibitor groups. Taken together, our data here suggest that bta-miR-370 has a negative regulation role for OLR1 both at the gene expression and protein levels and bovine adipocytes cytoplasm lipid droplets formation, which provides a reference for illustrating how the OLR1 gene affects milk production and beef quality traits in cattle.
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