Abstract-This paper presents C-MAC, a new MAC protocol designed to achieve high-throughput bulk communication for dataintensive sensing applications. C-MAC exploits concurrent wireless channel access based on empirical power control and physical interference models. Nodes running C-MAC estimate the level of interference based on the physical Signal-to-Interference-plusNoise-Ratio (SINR) model and adjust the transmission power accordingly for concurrent channel access. C-MAC employs a block-based communication mode that not only amortizes the overhead of channel assessment, but also improves the probability that multiple nodes within the interference range of each other can transmit concurrently. C-MAC has been implemented in TinyOS-1.x and extensively evaluated on Tmote nodes. Our experiments show that C-MAC significantly outperforms the state-of-art CSMA protocol in TinyOS with respect to system throughput, delay and energy consumption.
Abundant, codominant simple sequence repeats (SSRs) markers can be used for constructing genetic linkage maps and in marker-assisted breeding programs. Enrichment methods for SSR motifs were optimized with the ultimate aim of developing numerous loci in flowering dogwood (C. florida L.) genome. Small insert libraries using four motifs (GT, CT, TGG, and AAC) were constructed with C. florida 'Cherokee Brave' deoxyribonucleic acid (DNA). Colony polymerase chain reaction (PCR) of 2,208 selected clones with three primers we reported previously indicated that 47% or 1,034 of the clones harbored one of the four targeted SSR motifs. Sequencing the putative positive clones confirmed that nearly 99% (1,021 of 1,034) of them contained the desired motifs. Of the 871 unique SSR loci, 617 were dinucleotide repeats (70.8%), and 254 were trinucleotide or longer repeats (29.2%). In total, 379 SSR loci had perfect structure, 237 had interrupted, and 255 had compound structure. Primer pairs were designed from 351 unique sequences. The ability of the 351 SSR primer pairs to amplify specific loci was evaluated with genomic DNA of 'Appalachian Spring' and 'Cherokee Brave'. Of these primers, 311 successfully amplified product(s) with 'Cherokee Brave' DNA, 21 produced weak or faint products, and 19 did not amplify any products. Additionally, 218 of the 311 primers pairs revealed polymorphisms between the two cultivars, and 20 out of 218 primers detected an average of 13.7 alleles from 38 selected Cornus species and hybrids. These SSR loci constitute a valuable resource of ideal markers for both genetic linkage mapping and gene tagging of flowering dogwood.
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