We recently reported the use of a biphasic approach to generate topologically segregated bilayer beads. In generating 'one-bead one-compound' (OBOC) combinatorial libraries, novel encoding methods have been applied to these beads such as the testing library compound and the coding tags residing on the outer layer and inner core of each bead, respectively. In this report, we further exploit these bilayer beads by preparing target bead-libraries with low concentration of random peptides on the outer layer, and full substitution of coding peptides in the bead interior. The low concentration of peptide on the bead surface enables us to greatly increase the stringency of screening so that higher affinity ligands can easily be identified. Full substitution of the inner core of the beads enables us to have enough coding peptides inside the bead for direct microsequencing with Edman chemistry. The biphasic approach of preparing bilayer beads can be carried out at any point during the library construction. Therefore, the nonsequencable or fixed structures of the peptides can be bypassed in the coding tags. As a result, peptide libraries that otherwise cannot be sequenced can now be sequenced, and peptide segments with fixed residues within the libraries can be bypassed so that the microsequencing time can be significantly shortened. Furthermore, peptides with a branch of random sequence in the middle of a fixed peptide chain can be encoded with just the random sequence in the bead interior. We have successfully applied these novel OBOC library concepts in the optimization of cell-surface ligands for a human T-cell leukemia, Jurkat, cell line.

Canted-Cosine-Theta (CCT) magnet is an accelerator magnet that superposes fields of nested and tilted solenoids that are oppositely canted. The current distribution of any canted layer generates a pure harmonic field as well as a solenoid field that can be cancelled with a similar but oppositely canted layer. The concept places windings within mandrel's ribs and spars that simultaneously intercept and guide Lorentz forces of each turn to prevent stress accumulation. With respect to other designs, the need for pre-stress in this concept is reduced by an order of magnitude making it highly compatible with the use of strain sensitive superconductors such as Nb 3 Sn or HTS. Intercepting large Lorentz forces is of particular interest in magnets with large bores and high field accelerator magnets like the one foreseen in the future high energy upgrade of the LHC. This paper describes the CCT concept and reports on the construction of CCT1 a "proof of principle" dipole.

The HQ magnet is a 120 mm aperture, 1-meter-long Nb3Sn quadrupole developed by the LARP collaboration in the framework of the High-Luminosity LHC project. A first series of coils was assembled and tested in 5 assemblies of the HQ01 series. The HQ01e model achieved a maximum gradient of 170 T/m at 4.5 K at LBNL in 2010-2011 and reached 184 T/m at 1.9 K at CERN in 2012. A new series of coils incorporating major design changes was fabricated for the HQ02 series. The first model, HQ02a, was tested at Fermilab where it reached 98% of the short sample limit at 4.5 K with a gradient of 182 T/m in 2013. However, the full training of the coils at 1.9 K could not be performed due to a current limit of 15 kA. Following this test, the azimuthal coil pre-load was increased by about 30 MPa and an additional current lead was installed at the electrical center of the magnet for quench protection studies. The test name of this magnet changed to HQ02b. In 2014, HQ02b was then shipped to CERN as the first opportunity for full training at 1.9 K. In this paper, we present a comprehensive summary of the HQ02 test results including: magnet training at 1.9 K with increased pre-load, quench origin and propagation, and ramp rate dependence. A series of powering tests was also performed to assess changes in magnet performance with a gradual increase of the MIITs. We also present the results of quench protection studies using different setting for detection, heater coverage, energy extraction and the Coupling-Loss Induced Quench (CLIQ) system. However, the full training of the coils at 1.9 K could not be performed due to a current limit of 15 kA. Following this test, the azimuthal coil pre-load was increased by about 30 MPa and an additional current lead was installed at the electrical center of the magnet for quench protection studies. The test name of this magnet changed to HQ02b. In 2014, HQ02b was then shipped to CERN as the first opportunity for full training at 1.9 K. In this paper, we present a comprehensive summary of the HQ02 test results including: magnet training at 1.9 K with increased preload, quench origin and propagation, and ramp rate dependence. A series of powering tests was also performed to assess changes in magnet performance with a gradual increase of the MIITs. We also present the results of quench protection studies using different setting for detection, heater coverage, energy extraction and the Coupling-Loss Induced Quench (CLIQ) system.

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