A total of 720 newly-hatched Arbor Acres broilers were selected to investigate the effects of fructooligosaccharide (FOS) and B. subtilis on performance and microbial gut concentration in broilers. The result indicated that dietary supplementation with FOS or Bacillus in comparison to an antibiotic had selective effects on increasing caecal concentration of beneficial bacteria such as Lactobacillus and decreasing concentration of harmful bacteria such as E. Coli and Salmonella. However, supplemental aureomycin had non-selective effects on caecal microflora which inhibited all bacteria. Moreover, the combination of FOS and B. subtilis had much better improvement on caecal micro-ecosystem for broiler than they were used solely. Dietary addition of FOS or B. subtilis improved performance of broilers to the levels of those fed the diet with aureomycin and the combination of FOS and bacillus had better effects on reducing diarrhea rate and promoting growth than they were used solely.
Acetoin and 2,3-butanediol are commonly used as platform chemicals and widely used in pharmaceutical industries. 2,3-butanediol dehydrogenase/acetoin reductase (2,3-BDH/AR) plays a significant role in the microbial production of acetoin and 2,3-butanediol. In this study, 2,3-BDH was cloned from Corynebacterium crenatum SYPA5-5, was expressed in Escherichia coli BL21 and characterized with respect to the optimal temperature, pH, substrate specificity and kinetics. The results will guide further studies in Coryne. crenatum SYPA5-5 for the production of acetoin and 2,3-butanediol.
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