Excitatory pyramidal neurons in the entorhinal cortical layer II region (ECII PN ) directly innervate parvalbumin neurons in the CA1 hippocampus (CA1 PV ). (a) A brain section from ChR2 loxP/loxP mice injected with 1.2 μl of the AAV1/2-D28K-Cre virus particles in the ECII region and stained with DAPI. (b) High magnification of a selected area shows the ChR2-eGFP expression in ECII PN . (c) Whole-cell patch clamp recordings from ECII PN in a slice from the ECII PN ChR2+ mouse. Representative recordings of action potential firings in ECII PN in response to blue laser light illuminations in ECII PN . (d) The evoked EPSCs in CA1 PV in response to the increased intensities of blue laser lights in the slices from a ECII PN ChR2+ mouse. For more information on this topic, please refer to the article by Yang et al. on pages 199-210.
Recently, some studies suggested that UBQLN1 variant was associated with AD risk. However, the results were inconsistent. This meta-analysis aimed to determine the association between UBQLN1 variant and AD risk. We searched the electronic databases PubMed, Embase, and CNKI databases. Random-effects model was used. All statistical tests were performed using the STATA 11.0 software (StataCorp, College Station, TX, USA). UBQLN1 variant was not associated with the risk of AD (OR=1.05; 95%CI, 0.92-1.19; I2=35%). The corresponding pooled ORs were not materially altered in sensitivity analysis. The Galbraith plot was used to find the source of the heterogeneity and no study was the outlier. The shape of the funnel plot showed symmetry. Egger's test found no evidence of publication bias (P=0.8). These results suggest that the UBQ-8i polymorphism was not associated with AD risk.
Drosophila egg-derived tyrosine phosphatase (EDTP), a lipid phosphatase that removes 3position phosphate at the inositol ring, has dual functions in the oogenesis and the muscle performance during adult stages. A mammalian homologous gene MTMR14, which encodes the myotubularin-related protein 14, negatively regulates autophagy. Mutation of EDTP/MTMR14, however, causes at least three deleterious consequences: (1) lethality in the early embryogenesis in Drosophila; (2) "jumpy" phenotype with apparently impaired motor functions; and (3) association with a rare genetic disorder called centronuclear myopathy. Here we show that flies carrying a heterozygous EDTP mutation had increased survivorship to prolonged anoxia; tissuespecific downregulation of EDTP in non-muscle tissues, particularly motoneurons, extended the lifespan; and tissue-specific downregulation of EDTP in motoneurons improved the survivorship to beta-amyloid peptides (Aβ42) and polyglutamine (polyQ) protein aggregates. MTMR14 expression was evident in the hippocampus and cortex in C57BL/6J and APP/PS1 mice.Compared with C57BL/6J mice, APP/PS1 mice had reduced MTMR14 in the cortex but not in the hippocampus. Hippocampal expression of MTMR14 was increased and plateaued at 9-17 months compared with 2-6 months in C57BL/6J mice. Aβ42 treatment increased the expression of MTMR14 in the primarily cultured hippocampal neurons of Sprague/Dawley rats and mouse Neuro2a neuroblasts. We demonstrated a novel approach of tissue-specific manipulation of the disease-associated gene EDTP/MTMR14 for lifespan extension and the improvement of survivorship to cellular protein aggregates.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.