isms. 9 Hydroxypropyl-b-cyclodextrin (HP-b-CD), a CD derivative, has a greater capacity for complex formation than CD, and it has been recently used for the bioconversion of phytosterol to ADD and AD. 5,10 Recently, it was demonstrated that the bacterial strain Mycobacterium neoaurum NwIB-yV can be used in transforming phytosterol to 9-OH-AD. 11 The production of 9-OH-AD and the associated space-time yield reached 7.33 g L -1 and 1.22 g L -1 d -1 , respectively, from 15 g L -1 phytosterol in an aqueous system using growing M. neoaurum NwIB-yV cells, indicating a high potential for industrial appli-The steroid metabolites 9-hydroxy-androstenedione (9-OH-AD), androstadienedione (ADD) and androstenedione (AD) are important steroidal pharmaceuticals. In order to raise the production of steroid metabolites, an efficient resting cell phytosterol bioconversion process was developed to produce 9-OH-AD in the presence of hydroxypropyl-b-cyclodextrin (HP-b-CD). Cell growth medium containing phytosterol as an inducer positively improved cell activity. Under aerobic conditions, bioconversion proceeded at 70 g L -1 phytosterol in the presence of HP-b-CD (the optimized molar ratio of HP-b-CD/phytosterol was 1:1) with 30 g L -1 resting Mycobacterium neoaurum NwIB-yV cells (cell dry mass) in a 5-L bioreactor, where 9-OH-AD production and space-time yield reached 36.4 g L -1 and 9.1 g L -1 d -1 , respectively. The recycling of cells and HP-b-CD enables cost-saving and industrial applications. This bioprocess was also applied for the production of ADD and AD. The production of these steroid metabolites was much higher than that reported in previous studies.
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