Long-term alcohol intake from food can lead to numerous mental disorders in humans, and cause serious problems for governments and families worldwide. However, currently, it is unclear how alcohol affects the hypothalamic–pituitary–adrenal (HPA) axis. In the present study, using zebrafish larvae exposed to 1% ethanol, we made Zebrafish behavioural analysis, samples were collected for the enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR) experiments,and made statistical analyses at last. we found that ethanol decreases the locomotor activity of zebrafish larvae,showed a more intense reaction to external stimuli,increases the secretion of HPA axis hormones in zebrafish larvae,influences the secretion of neurotransmitters,alters key gene expression during neurotransmitter metabolism. Ethanol exposure reduced zebrafish locomotor activity, increased their HPA axis activity, and led to significant changes in the secretion of dopamine and serotonin. These findings provide us with a new understanding of the effects of ethanol on the HPA axis.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.