Excessive nitrogen (N) fertilizer application and poor timing of N fertilizer application to winter wheat are common problems on the North China Plain. To study the possibilities of optimizing the timing and rate of N application, a field experiment was conducted from 1999 to 2001 in a suburb of Beijing. A control (no nitrogen) and two N fertilization strategies (conventional N application and optimized N fertilization) were designed to compare their effects on wheat growth, N nutrient status, grain yield and N balance. The conventional N fertilization strategy was given a fixed N rate of 300 kg N ha À1 , which was split, half in autumn and half in spring as a top-dressing. The timing and rate of N and application of the optimized N fertilization strategy were determined by the target value of soil mineral nitrogen demand for three growth periods of wheat, which is related to the target yield, and soil mineral N (N min ) in the effective rooting depth at the beginning of these three periods. Based on the optimized N fertilization strategy, a total of 55 and 65 kg N ha À1 had to be applied to winter wheat in the re-greening and shooting stages of the first and second experimental years, respectively. Compared with the high N rate before sowing in the conventional N fertilization treatment, the optimized N fertilization treatment did not require any N fertilizer before sowing of wheat. Despite a much lower N fertilization rate, no significant difference in N nutrient status, growth during the wheat growing period or grain yield was observed between optimized N and conventional N fertilization treatments. As a consequence of optimizing the rate and timing of the N fertilizer application to match wheat demand, a much lower residual N min and calculated apparent N loss was found as compared to the conventional N treatment. N recovery for the optimized N fertilization treatment (67% in 1999/2000 and 66% in 2000/2001) was much higher than that of the conventional N fertilization treatment (19% in 1999/2000 and 18% in 2000/2001). In conclusion, the optimized N fertilization strategy can synchronize N demand of wheat and the N supply from soil and fertilizer, and therefore drastically reduce N application rates without any yield losses.
All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher.
The cotton aphid, Aphis gossypii, is one of the most economically important agricultural pests worldwide as it is polyphagous and resistant to many classes of insecticides. Overexpression of the cytochrome P450 monooxygenase (P450) CYP6DA2 has previously been found to be associated with gossypol and spirotetramat tolerance in the cotton aphid. In the present study, the elements located in the promoter region (-357:-343; -250:-241; -113:-104) of CYP6DA2 were shown to control promoter activity, and gossypol induction was observed. We hypothesized that the expression of CYP6DA2 is subject to transcriptional regulation. To investigate the underlying mechanism, we assessed two transcription factors, aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT), and found that the abundance of AhR was highly correlated with CYP6DA2 abundance. RNA interference of AhR or ARNT significantly decreased the levels of the target gene as well as those of its counterpart, and both dramatically repressed CYP6DA2 expression. Cotransfection of the ARNT, AhR, or AhR plus ARNT and CYP6DA2 promoter constructs elevated CYP6DA2 promoter activity, with the AhR plus ARNT cotransfection being the most effective. Thus, these elements located in the promoter were responsible for CYP6DA2 transcription, and CYP6DA2 expression was regulated by the transcription factors AhR and ARNT.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.