Cleavage efficiency plays a key role in clustered regularly interspaced short palindromic repeat (
CRISPR
)‐based gene editing, particularly when the given guide
RNA
exhibits low cleavage activity. Here, we describe the packaging of tandem guide
RNA
s and single‐strand annealing‐based surrogate reporter cassettes into the
CRISPR
/CRISPR‐associated protein 9 vector, which increased gene‐editing efficiency by 4.94–6.31‐fold and simultaneously enriched the proportion of genetically modified cells. This strategy may substantially improve genome‐editing efficiency for demanding applications.
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