Aqueous phospholipid preparations comprised of 1,2‐dimyristoyl‐sn‐glycero‐3‐phosphocholine (DMPC) and 1,2‐dihexanoyl‐sn‐glycero‐3‐phosphocholine (DHPC) are prevalent materials for biological characterization and become gel‐like near physiological temperature, but have a low viscosity below 24°C. The rheology of 20% phospholipid preparations of [DMPC]/[DHPC] = 2.5 reveals that, under conditions utilized for fluid steering, the materials are shear‐thinning power‐law fluids with a power‐law index ranging from 0.30 through 0.90. Phospholipid preparations are utilized to steer fluids in microfluidic chips and support hydrodynamic delivery of sample across a double T injection region in a chip. The fact that the phospholipids are fully integrated as a valving material as well as a separation medium is demonstrated through the separation of linear oligosaccharides labeled with 1‐aminopyrene‐3,6,8‐trisulfonic acid.
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