SUMMARY
A centrifugation method for depositing cells on cover‐glasses for scanning electron microscopy (SEM) is described. This centrifugation procedure provides a defined and standardized morphology of mononuclear cells from human blood. The method circumvents the highly variable flattening of some blood cells such as monocytes, observed with some methods. The SEM images show fine morphological surface details indicating a well‐preserved cell morphology. The cell recovery of the method is sufficiently high and the lymphocyte‐monocyte ratio in centrifugation preparations was found identical to the ratio in control smear preparations.
A method is described in which the surface morphology of benign and malignant cervical cells is investigated with a combined light microscope-scanning electron microscope, after the measurement of the DNA content of each individual cell in the same instrument. The suspect cells can thus be identified by an increased aneuploid DNA content (greater than 5C) and not primarily by morphology. The DNA content was measured, after a quantitative acriflavine-Feulgen staining, by using a microphotometer attached to the combined microscope. It was found that the suspect cells show a different surface morphology compared to normal cells from a benign specimen.
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