Background/Aim: Breast cancer is one of the most common cancers in women all over the world and new treatment options are urgent. ER stress in cancer cells results in apoptotic cell death, and it is being proposed as a new therapeutic target. SH003, a newly developed herbal medicine, has been reported to have anti-cancer effects. However, its molecular mechanism is not yet clearly defined. Materials and Methods: Microarray was performed to check the differential gene expression patterns in various breast cancer cell lines. Cell viability was measured by MTT assays to detect cytotoxic effects. Annexin V-FITC and 7AAD staining, TUNEL assay and DCF-DA staining were analyzed by flow cytometry to evaluate apoptosis and ROS levels, respectively. Protein expression was examined in SH003-breast cancer cells using immunoblotting assays.
The expression of C/EBP Homologous Protein (CHOP) mRNA was measured by real-time PCR. The effects of CHOP by SH003 treatment were investigated using transfection method. Results: Herein, we investigated the molecular mechanisms through which SH003 causes apoptosis of human breast cancer cells. Both cell viability and apoptosis assays confirmed the SH003-induced apoptosis of breast cancer cells. Meanwhile, SH003 alteredthe expression patterns of several genes in a variety of breast cancer cell lines. More specifically, it upregulated gene sets including the response to unfolded proteins, independently of the breast cancer cell subtype. In addition, SH003-induced apoptosis was due to an increase in ROS production and an activation of the ER stress-signaling pathway. Moreover, CHOP gene silencing blocked SH003induced apoptosis. Conclusion: SH003 causes apoptosis of breast cancer cells by upregulating ROS production and activating the ER stress-mediated pathway. Thus, our findings suggest that SH003 can be a potential therapeutic agent for breast cancer.
This research aims to identify the motivations behind China’s refugee policy through a comparative historical analysis of the mass influx of refugees from two neighboring states: Vietnam, starting in the late 1970s, and North Korea starting in the 1990s. The study argues that similar motivations, grounded in China’s concerns regarding sovereignty, national security and the regional balance of power, generated contrasting responses to the two groups of refugees. This research briefly reviews the causes, development and nature of the two refugee crises, and then analyzes China’s Janus-faced refugee policy with its two different responses. Last, this research indicates China’s recent positive engagements with refugee issues and emphasizes the significance of China’s role in regional refugee protection.
Google Maps have changed the response time of Web-GIS using AJAX technologies.In addition, Google released the Open API named Google Maps API(Application Programming Interface) and it lead to the big paradigm on the Open API, where the SDK(Software Development Kit) and ASP(Application Service Provider) had ruled at the related map market. In short, the Open API has been paradigm-shifting for the web mapping. After this, government, many companies and open source foundations have guided Web-GIS market's growth through releasing the relevant Open APIs. So many comparative analysis on web-mapping API carried out by many researches. However there were no researches that can be applied to our current domestic environments. This paper investigates components of web-mapping API. Then we compare how many components supported and enumerate features for each of those APIs. Finally this paper presents direction of future development of Web Mapping API.
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