The denatured state of a protein contains important information about the determinants of the folding process. By combining site-directed spin-labeling NMR experiments and restrained computer simulations, we have determined ensembles of conformations that represent the denatured state of the bovine acyl-coenzyme A binding protein (ACBP) at three different concentrations of guanidine hydrochloride. As the experimentally determined distance information corresponds to weighted averages over a broad ensemble of structures, we applied the experimental restraints to a system of noninteracting replicas of the protein by using a Monte Carlo sampling scheme. This procedure permits us to sample ensembles of conformations that are compatible with the experimental data and thus to obtain information regarding the distribution of structures in the denatured state. Our results show that the denatured state of ACBP is highly heterogeneous. The high sensitivity of the computational method that we present, however, enabled us to identify long-range interactions between two regions, located near the N- and C-termini, that include both native and non-native elements. The preferential formation of these contacts suggests that the sequence-dependent patterns of helical propensity and hydrophobicity are important determinants of the structure in the denatured state of ACBP.
A new class of water-soluble, amphiphilic star block copolymers with a large number of arms was
prepared by sequential atom transfer radical polymerization (ATRP) of n-butyl methacrylate (BMA) and poly(ethylene glycol) methyl ether methacrylate (PEGMA). As the macroinitiator for the ATRP, a 2-bromoisobutyric
acid functionalized fourth-generation hyperbranched polyester (Boltorn H40) was used, which allowed the
preparation of star polymers that contained on average 20 diblock copolymer arms. The synthetic concept was
validated by AFM experiments, which allowed direct visualization of single molecules of the multiarm star block
copolymers. DSC and SAXS experiments on bulk samples suggested a microphase-separated structure, in agreement
with the core−shell architecture of the polymers. SAXS experiments on aqueous solutions indicated that the star
block copolymers can be regarded as unimolecular micelles composed of a PBMA core and a diffuse PPEGMA
corona. The ability of the polymers to encapsulate and release hydrophobic guests was evaluated using 1H NMR
spectroscopy. In dilute aqueous solution, these polymers act as unimolecular containers that can be loaded with
up to 27 wt % hydrophobic guest molecules.
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