Several members of the genus Methanosarcina were investigated by room‐temperature and low‐temperature difference spectroscopy for the presence of cytochromes. In combination with potentiometric titrations two membrane‐bound b‐cytochromes and one membrane‐bound c‐cytochrome could be detected in cells grown on methanol or trimethylamine. Very probably acetate‐grown cells contained an additional cytochrome b. The midpoint potentials of the two b‐type cytochromes were Em1=−325 mV and Em2=−183 mV, respectively. The additional b cytochrome formed during growth on acetate exhibited a midpoint potential of Em3=−250 mV.
During three and a half years of psychiatric liaison with a cardiac transplantation program, the authors evaluated and followed 101 transplantation candidates. Ten exhibited pronounced ambivalence toward cardiac transplantation. Ambivalence appears to be associated with three factors: difficulties facing the seriousness of the cardiac illness, fear of the actual operation, and quality of life-concerns. An essentially nonpathological response, ambivalence can produce significant psychiatric symptoms and management problems in transplant candidates. Its resolution can be facilitated by psychotherapy, appropriate medication, peer support, and patience. Some patients may need particular assistance in understanding that refusal of transplantation is an acceptable alternative.
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