(250 words) 18Under conditions of nutrient-limited growth, Type II obligate methanotrophs oxidize C 1 19 compounds, such as methane or methanol and accumulate intracellular granules of poly(3-20 hydroxybutyrate) (P3HB). Here, we report that, under same nutrient-limited conditions, the Type 21 II obligate methanotroph Methylocystis parvus OBBP can use ethane as its sole carbon and 22 energy source for synthesis P3HB granules, accumulating up to 35 ± 4 wt% P3HB.13 C-NMR 23 spectra of the P3HB confirmed incorporation of 13 C from [ 13 C 2 ]ethane. Moreover, when valerate 24 was added as a co-substrate with ethane, oxidation of the ethane supported synthesis of the 25 copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). 26 IMPORTANCE (150 words, nontechnical explanation of the significance of the study) 27The presence of ethane in natural gas is often considered undesirable for methanotroph-based 28 biotechnology due to the C 1 specialization of obligate methanotrophs and concerns about 29 inhibitory byproducts arising from methane monooxygenase-mediated cometabolism of ethane. 30This work establishes that co-oxidation of ethane and further metabolism in the absence of 31 methane can support synthesis of the valuable polyhydroxyalkanoate bioplastics P3HB and 32 PHBV. 33KEYWORDS 34 ethane; methanotroph; methane monooxygenase; PHA; PHB; natural gas; polyhydroxyalkanoate 35 synthesis 36 37 All rights reserved. No reuse allowed without permission.
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