The effects of dietary conjugated linoleic acid (CLA) supplementation on growth performance, carcass quality, free fatty acid composition, and accumulation of CLA were investigated in broilers. Four hundred eighty 3-wk-old broilers were assigned to 4 dietary treatments (0, 0.5, 1.0, and 1.5% CLA) with 6 replicates of 20 chickens. Feed consumption and BW were recorded at every 5-d period. On d 42, carcass compositions were determined from 30 birds per treatment. There were no significant differences in average daily feed intake among the treatments. However, feed conversion ratio was highly significantly different (P < 0.01) between treatments, and average daily gain was significantly reduced by a supplement of dietary CLA (P < 0.05). Live weight and percentages of carcass, drumstick, thigh, boneless thigh, pectoralis major, and pectoralis minor were not influenced by the dietary CLA. Abdominal fat was significantly reduced (P < 0.01) with the increased CLA level in the broilers' diets. Percentages of liver weight were significantly increased (P < 0.01) after the CLA treatments. Percentages of drumstick and boneless drumstick were significantly decreased (P < 0.05) by dietary CLA. In terms of free fatty acid composition in broiler meat, CLA addition significantly increased (P < 0.05) saturated fatty acids, especially in thigh muscle, whereas unsaturated fatty acids were unchanged. Accumulations of CLA in meat were significantly increased (P < 0.05) with increasing CLA level in the diet.

The experiment was conducted to determine the effects of cutting interval and cutting height on the yield and nutrient composition of King Napier grass (Pennisetum purpureum x Pennisetum americanum) when grown on a sandy soil in the Northeast of Thailand. The cutting intervals were 30, 45 and 60 days between harvests and the cutting heights were 5, 10 and 15 cm above ground level. The experiment was a 3×3 factorial layout in a randomized complete block design with 4 replications-giving a total of 36 plots each 3×3 m 2 . Harvested plant material was weighed, dried and the ground subsamples taken for analyses of crude protein (CP), crude fiber (CF), ash, ether extract (EE), acid detergent fiber (ADF), neutral detergent fiber (NDF), acid detergent lignin (ADL), Hemicellulose and Cellulose. Results showed that increasing the cutting interval (i.e. advancing age of maturity) increased dry matter and nutrient yields significantly. In terms of nutrient content, it also increased the crude fiber, acid detergent fiber (ADF), neutral detergent fiber (NDF) and acid detergent lignin (ADL) percent in the plant. However, crude protein and ash percent was markedly decreased as the cutting interval increased. Increasing cutting height had no effect on dry matter yield and yields of nutrients, but in terms of nutrient content, it decreased crude fiber content. The percent EE, NDF, ADF, Hemicellulose and Cellulose in the plant was unaffected by cutting height. From the results presented it is clear that cutting a stand of King Napier grass every 45 to 60 days will achieve greater dry matter and nutrient yields than cutting more frequently, at 30 days. The cutting height at harvest, whether 5, 10 or 15 cm above ground level had no effect on dry matter or nutrient yields of King Napier grass.

Conjugated linoleic acid (CLA) is a mixture of positional and geometric isomers of linoleic acid with conjugated double bonds. Conjugated linoleic acids have been reported to have a wide range of health-beneficial effects, including anticarcinogenic, antiatherogenic, antidiabetic, and immune stimulatory effects. The objective of this study was to investigate the incorporation of CLA into eggs. Three hundred 27-wk-old layers were allocated to 5 dietary treatments (0, 1, 2, 3, and 4% CLA) with 5 replicates. The results of the study showed that average daily feed intakes were similar in all treatment groups, although hens fed with 4% CLA tended to consume less feed than other hens. Body weight gain and mortality rate were not significantly different (P > 0.05). Hens fed 4% dietary CLA had reduced egg, yolk, and albumen weights (P < 0.05). Yolk color significantly decreased as dietary CLA increased (P < 0.01). Shell thickness and Haugh units were not influenced by the dietary CLA. Concentrations of CLA and saturated fatty acids in egg yolk lipids increased as dietary CLA increased (P < 0.01), whereas concentrations of monounsaturated fatty acids and polyunsaturated fatty acids decreased as dietary CLA increased (P < 0.01). It can be concluded from the present experiments that increasing the amount of CLA fed to hens will increase the amount of CLA in egg yolk and that this increase is accompanied by a reduction in the amount of yolk polyunsaturated fatty acids but an increase in yolk saturated fatty acids. Egg size, yolk weight, and Roche-fan determined yolk color significantly decreased at the highest level of CLA supplementation.

No abstract

To evaluate the effects of feeding linseed oil or/and sunflower oil mixed with fish oil on milk yield, milk composition and fatty acid (FA) profiles of dairy cows fed a high-concentrate diet, 24 crossbred primiparous lactating dairy cows in early lactation were assigned to a completely randomized design experiment. All cows were fed a high-concentrate basal diet and 0.38 kg dry matter (DM) molasses per day. Treatments were composed of a basal diet without oil supplement (Control), or diets of (DM basis) 3% linseed and fish oils (1:1, w/w, LSO-FO), or 3% sunflower and fish oils (1:1, w/w, SFO-FO), or 3% mixture (1:1:1, w/w) of linseed, sunflower, and fish oils (MIX-O). The animals fed SFO-FO had a 13.12% decrease in total dry matter intake compared with the control diet (p<0.05). No significant change was detected for milk yield; however, the animals fed the diet supplemented with SFO-FO showed a depressed milk fat yield and concentration by 35.42% and 27.20%, respectively, compared to those fed the control diet (p<0.05). Milk c9, t11-conjugated linoleic acid (CLA) proportion increased by 198.11% in the LSO-FO group relative to the control group (p<0.01). Milk C18:3n-3 (ALA) proportion was enhanced by 227.27% supplementing with LSO-FO relative to the control group (p<0.01). The proportions of milk docosahexaenoic acid (DHA) were significantly increased (p<0.01) in the cows fed LSO-FO (0.38%) and MIX-O (0.23%) compared to the control group (0.01%). Dietary inclusion of LSO-FO mainly increased milk c9, t11-CLA, ALA, DHA, and n-3 polyunsaturated fatty acids (PUFA), whereas feeding MIX-O improved preformed FA and unsaturated fatty acids (UFA). While the lowest n-6/n-3 ratio was found in the LSO-FO, the decreased atherogenecity index (AI) and thrombogenicity index (TI) seemed to be more extent in the MIX-O. Therefore, to maximize milk c9, t11-CLA, ALA, DHA, and n-3 PUFA and to minimize milk n-6/n-3 ratio, AI and TI, an ideal supplement would appear to be either LSO-FO or MIX-O.

The objective of this study was to determine the effect of palm and/or linseed oil (LSO) supplementation on carcass quality, sensory evaluation and fatty acid profile of beef from crossbred Wagyu beef steers. Twenty four fattening Wagyu crossbred beef steers (50% Wagyu), averaging 640±18 kg live weight (LW) and approximately 30 mo old, were stratified and randomly assigned in completely randomized design into 3 treatment groups. All steers were fed approximately 7 kg/d of 14% crude protein concentrate with ad libitum rice straw and had free access to clean water and were individually housed in a free-stall unit. The treatments were i) control concentrate plus 200 g/d of palm oil; ii) control concentrate plus 100 g/d of palm oil and 100 g/d of LSO, iii) control concentrate plus 200 g/d of LSO. This present study demonstrated that supplementation of LSO rich in C18:3n-3 did not influence feed intakes, LW changes, carcass and muscle characteristics, sensory and physical properties. LSO increased C18:3n-3, C22:6n-3, and n-3 polyunsaturated fatty acids (PUFA), however, it decreased C18:1t-11, C18:2n-6, cis-9, trans-11, and trans-10,cis-12 conjugated linoleic acids, n-6 PUFA and n-6:n-3 ratio in Longissimus dorsi and Semimembranosus muscles.

The objective of this study was to determine the effects of linseed oil or whole linseed supplementation on performance and milk fatty acid composition of lactating dairy cows. Thirty six Holstein Friesian crossbred lactating dairy cows were blocked by milking days first and then stratified random balanced for milk yields and body weight into three groups of 12 cows each. The treatments consisted of basal ration (53:47; forage:concentrate ratio, on a dry matter [DM] basis, respectively) supplemented with 300 g/d of palm oil as a positive control diet (PO), or supplemented with 300 g/d of linseed oil (LSO), or supplemented with 688 g/d of top-dressed whole linseed (WLS). All cows were received ad libitum grass silage and individually fed according to the treatments. The experiment lasted for 10 weeks including the first 2 weeks as the adjustment period, followed by 8 weeks of measurement period. The results showed that LSO and WLS supplementation had no effects on total dry matter intake, milk yield, milk composition, and live weight change; however, the animals fed WLS had higher crude protein (CP) intake than those fed PO and LSO (p<0.05). To compare with the control diet, dairy cow’s diets supplemented with LSO and WLS significantly increased milk concentrations of cis-9, trans-11-conjugated linoleic acid (CLA) (p<0.05) and n-3 fatty acids (FA) (p<0.01), particularly, cis-9,12,15-C18:3, C20:5n-3 and C22:6n-3. Supplementing LSO and WLS induced a reduction of medium chain FA, especially, C12:0-C16:0 FA (p<0.05) while increasing the concentration of milk unsaturated fatty acids (UFA) (p<0.05). Milk FA proportions of n-3 FA remarkably increased whereas the ratio of n-6 to n-3 decreased in the cows supplemented with WLS as compared with those fed the control diet and LSO (p<0.01). In conclusion, supplementing dairy cows’ diet based on grass silage with WLS had no effect on milk yield and milk composition; however, trans-9- C18:1, cis-9, trans-11-CLA, n-3 FA and UFA were increased while saturated FA were decreased by WLS supplementation. Therefore, it is recommended that the addition 300 g/d of oil from whole linseed should be used to lactating dairy cows’ diets.

No abstract