Makromolekul dan Hasil Alam merupakan salah satu praktikum yang dilayani UPT.Laboratorium Terpadu dan merupakan praktikum yang baru, sehingga untuk memenuhi tujuan pembelajaran perlu dilakukan uji coba objek praktikum yang sesuai. Tujuan penelitian ini adalah untuk mendapatkan kondisi yang memungkinkan dari pengujian senyawa flavonoid total agar dapat digunakan sebagai objek praktikum yang efektif dan efisien. Pada penelitian ini dilakukan optimasi kondisi pengujian senyawa flavonoid total dalam ekstrak daun katuk, pandan dan mengkudu dengan metode spectrofotometri yang menggunakan pereaksi AlCl3. Adapun parameter yang digunakan adalah pengaruh volume dan konsentrasi AlCl3, CH3COONa, waktu reduksi dan berat sampel terhadap kestabilan warna senyawa kompleks AlCl3. Dari hasil penelitian ini diperoleh kondisi optimum AlCl3dengan volume 0,1 mLkonsentrasi 5%, dan CH3COONa 0,2 mL konsentrasi 1M. Sedangkan waktu reduksi optimum adalah 45 menit, dan berat optimum ekstrak daun berkisar antara 50 mg-700 mg.
The problem studied in this study is the Community Satisfaction Index for service units of government agencies in the Regulation of the Minister of State for Administrative Reform and Bureaucratic Reform of the Republic of Indonesia Number 14 of 2017 at the UPT Library of Sriwijaya University. The purpose of this study was to determine the Community Satisfaction Index in library services, especially the Sriwijaya University institutional repository service. The method used in this research is descriptive quantitative. The research subjects were users of Sriwijaya University institutional repository services as many as 98 respondents obtained using the slovin formula. The sampling technique used is accidental sampling. Respondents were selected randomly according to service coverage. Data collection techniques using questionnaires and observation. The results of the study using the community satisfaction index method, especially in institutional repository services at the Sriwijaya University Library UPT, found that institutional repository services were considered very good. The results showed that the Community Satisfaction Index for institutional repository services at the Sriwijaya University Library UPT was 89.88 so that the quality of service was A.
Rice husks contain cellulose as a raw material for manufacturing second-generation bioethanol. Cellulose from pre-treated rice husks was converted into reducing sugars through enzymatic hydrolysis using enzymes derived from Aspergillus niger. This study aims to determine the kinetics of enzymatic hydrolysis at enzyme concentrations of 10, 15, and 20% (v/w) and hydrolysis times of 5, 10, 15, 20, and 25 h. The results showed that cellulose was hydrolyzed to form reducing sugars. The CMCase activity and FPase activity reached 548.940 and 314.892 U mL−1, respectively, much higher than most previous reports on this genus. From the calculation of the reaction rate using the Michaelis–Menten kinetic model, the value of the Michaelis constant ranges from 0.001 to 0.0007, and the maximum rate is 1.3 × 10−7 to 2.7 × 10−7 Mol L−1 s−1. The highest reducing sugar concentration was obtained (1.80 g L−1) at an enzyme concentration of 20% (v/w) and a hydrolysis time of 25 h.
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