Plaque accumulation on internal surface of denture is a common problem among removable denture wearers. Poor denture cleanliness can increase colonization of Candida albicans and cause inflammatory reaction of denture-bearing mucosa, i.e. denture stomatitis. Objective: To find out the effect of denture cleanliness level on denture stomatitis on maxillary denture-bearing mucosa in a group of removable denture wearers who received prosthodontic treatment at Poliklinik Gigi RSMH Palembang and to investigate the denture hygiene habits of removable denture wearers. Methods: Thirty subjects participated in this study. Denture cleanliness level was assessed with disclosing solution to disclose denture plaque on internal surface of maxillary denture. Cleanliness level was graded according to Budtz-Jorgensen. Intraoral examination was done to determine any visible signs of denture stomatitis. Data referring to denture hygiene habits of removable denture wearers was collected from interview using questionnaire. Data were analyzed using the Komolgorov-Smirnov test. Results: Result of the study showed that 40% subjects had poor upper denture cleanliness. Denture stomatitis was observed on maxillary denture-bearing mucosa in 43.3% subjects. Kolmogorov-Smirnov test showed that there was a significant effect of denture cleanliness level on denture stomatitis on maxillary denture-bearing mucosa (p<0.05). Conclusion: Denture cleanliness level influence the occurence of denture stomatitis on maxillary denture bearing-mucosa in a group of removable denture wearers who received prosthodontic treatment. ABSTRAKTingkat kebersihan gigi tiruan mempengaruhi terjadinya denture stomatitis pada mukosa pendukung gigi tiruan rahang atas. Akumulasi plak pada permukaan internal basis gigi tiruan lepas merupakan masalah umum yang dapat ditemukan pada pemakai gigi tiruan lepas. Kebersihan gigi tiruan yang kurang adekuat dapat meningkatkan kolonisasi Candida albicans dan dapat menyebabkan reaksi peradangan pada mukosa pendukung gigi tiruan yaitu denture stomatitis. Tujuan: Menganalisis pengaruh tingkat kebersihan gigi tiruan terhadap terjadinya denture stomatitis pada mukosa pendukung gigi tiruan rahang atas (RA) pada pasien yang menerima perawatan gigi tiruan lepas di Poliklinik Gigi RS Moh. Hoesin (RSMH) Palembang serta untuk mengetahui bagaimana cara pasien dalam menjaga kebersihan gigi tiruan lepas. Metode: Subjek penelitian berjumlah sebanyak 30 orang. Tingkat kebersihan gigi tiruan diukur melalui pewarnaan plak pada permukaan internal basis gigi tiruan RA dengan menggunakan disclosing solution dan dinilai dengan menggunakan kriteria kebersihan gigi tiruan Budtz-Jorgensen. Pemeriksaan klinis dilakukan untuk melihat ada atau tidaknya denture stomatitis. Data mengenai cara pasien dalam menjaga kebersihan gigi tiruan lepas didapat dari hasil wawancara. Data dianalisis menggunakan uji alternatif Kolmogorov-Smirnov. Hasil: 40% subjek penelitian memiliki kebersihan gigi tiruan RA yang buruk. Denture stomatitis ditemukan di mukosa pendukung...