Objective We investigated the effect of two different saline solutions on the mechanisms of
injury after intestinal ischemia: oxidative stress and inflammatory responses.Methods Wistar rats underwent transient superior mesenteric artery occlusion and were
studied for 6 hours after reperfusion. After randomization, the animals were
divided into four groups: Sham; Hypertonic Saline, in which they received infusion
of 4mL/kg body weight of 7.5% hypertonic saline; Saline, in which they received
infusion of 33mL/kg body weight of 0.9% saline; and Non Treatment. The infusion
was performed immediately prior to the reperfusion. The plasma concentrations of
interleukin 6 and interleukin 10 were measured. Tissue samples (lung, liver, and
intestine) were collected for malondialdehyde, myeloperoxidase, and interleukin
measurements.Results The animals that received infusions (Hypertonic Saline and Saline) showed lower
levels of tissue malondialdehyde, myeloperoxidase, interleukin 6, and interleukin
10 compared with the Non Treatment group. The plasma concentrations of interleukin
6 and interleukin 10 were higher in the animals treated with 7.5% hypertonic
saline compared with Saline and Non Treatment groups.Conclusion In this model of transient intestinal ischemia, the adequate maintenance of
intravascular volume decreased oxidative stress and the synthesis of inflammatory
markers. Both 7.5% Hypertonic Saline and Saline attenuated the deleterious effects
observed after intestinal ischemia.
Chimabucuro WK. Intestinal ischemia and intravascular fluid reposition. How two saline solutions containing different sodium chloride concentration could modify the deleterious responses triggered by intestinal reperfusion. An experimental model in rat [thesis].
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