BACKGROUND Ebola virus has been detected in the semen of men after their recovery from Ebola virus disease (EVD). We report the presence of Ebola virus RNA in semen in a cohort of survivors of EVD in Sierra Leone. METHODS We enrolled a convenience sample of 220 adult male survivors of EVD in Sierra Leone, at various times after discharge from an Ebola treatment unit (ETU), in two phases (100 participants were in phase 1, and 120 in phase 2). Semen specimens obtained at baseline were tested by means of a quantitative reverse-transcriptase–polymerase-chain-reaction (RT-PCR) assay with the use of the target sequences of NP and VP40 (in phase 1) or NP and GP (in phase 2). This study did not evaluate directly the risk of sexual transmission of EVD. RESULTS Of 210 participants who provided an initial semen specimen for analysis, 57 (27%) had positive results on quantitative RT-PCR. Ebola virus RNA was detected in the semen of all 7 men with a specimen obtained within 3 months after ETU discharge, in 26 of 42 (62%) with a specimen obtained at 4 to 6 months, in 15 of 60 (25%) with a specimen obtained at 7 to 9 months, in 4 of 26 (15%) with a specimen obtained at 10 to 12 months, in 4 of 38 (11%) with a specimen obtained at 13 to 15 months, in 1 of 25 (4%) with a specimen obtained at 16 to 18 months, and in no men with a specimen obtained at 19 months or later. Among the 46 participants with a positive result in phase 1, the median baseline cycle-threshold values (higher values indicate lower RNA values) for the NP and VP40 targets were lower within 3 months after ETU discharge (32.4 and 31.3, respectively; in 7 men) than at 4 to 6 months (34.3 and 33.1; in 25), at 7 to 9 months (37.4 and 36.6; in 13), and at 10 to 12 months (37.7 and 36.9; in 1). In phase 2, a total of 11 participants had positive results for NP and GP targets (samples obtained at 4.1 to 15.7 months after ETU discharge); cycle-threshold values ranged from 32.7 to 38.0 for NP and from 31.1 to 37.7 for GP. CONCLUSIONS These data showed the long-term presence of Ebola virus RNA in semen and declining persistence with increasing time after ETU discharge. (Funded by the World Health Organization and others.)

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-The thalamus is globally connected with distributed cortical regions, yet the functional significance of this extensive thalamocortical connectivity remains largely unknown. By performing graph-theoretic analyses on thalamocortical functional connectivity data collected from human participants, we found that the human thalamus displays network properties capable of integrating multimodal information across diverse cortical functional networks. From a metaanalysis of a large dataset of functional brain imaging experiments, we further found that the thalamus is involved in multiple cognitive functions. Finally, we found that focal thalamic lesions in humans have widespread distal effects, disrupting the modular organization of cortical functional networks. This converging evidence suggests that the human thalamus is a critical hub region that could integrate heteromodal information and maintain the modular structure of cortical functional networks.

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Abstract. With the wider use of ontologies in the Semantic Web and as part of production systems, multiple scenarios for ontology maintenance and evolution are emerging. For example, successive ontology versions can be posted on the (Semantic) Web, with users discovering the new versions serendipitously; ontology-development in a collaborative environment can be synchronous or asynchronous; managers of projects may exercise quality control, examining changes from previous baseline versions and accepting or rejecting them before a new baseline is published, and so on. In this paper, we present different scenarios for ontology maintenance and evolution that we have encountered in our own projects and in those of our collaborators. We define several features that categorize these scenarios. For each scenario, we discuss the high-level tasks that an editing environment must support. We then present a unified comprehensive set of tools to support different scenarios in a single framework, allowing users to switch between different modes easily. Evolution of Ontology EvolutionAcceptance of ontologies as an integral part of knowledge-intensive applications has been growing steadily. The word ontology became a recognized substrate in fields outside the computer science, from bioinformatics to intelligence analysis. With such acceptance, came the use of ontologies in industrial systems and active publishing of ontologies on the (Semantic) Web. More and more often, developing an ontology is not a project undertaken by a single person or a small group of people in a research laboratory, but rather it is a large project with numerous participants, who are often geographically distributed, where the resulting ontologies are used in production environments with paying customers counting on robustness and reliability of the system.The Protégé ontology-development environment 1 has become a widely used tool for developing ontologies, with more than 50,000 registered users. The Protégé group works closely with some of the tool's users and we have a continuous stream of requests from them on the features that they would like to have supported in terms of managing and developing ontologies collaboratively. The configurations for collaborative development differ significantly however. For instance, Perot Systems 2 uses a client-server mode of Protégé with multiple users simultaneously accessing the same copy of the ontology on the server. The NCI Center for Bioinformatics, which develops the NCI The-1 http://protege.stanford.edu 2

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BackgroundAfter the launch of Janani Suraksha Yojana, a conditional cash transfer scheme in India, the proportion of women giving birth in institutions has rapidly increased. However, there are important gaps in quality of childbirth services during institutional deliveries. The aim of this intervention was to improve the quality of childbirth services in selected high caseload public health facilities of 10 districts of Rajasthan. This intervention titled “Parijaat” was designed by Action Research & Training for Health, in partnership with the state government and United Nations Population Fund.MethodsThe intervention was carried out in 44 public health facilities in 10 districts of Rajasthan, India. These included district hospitals (9), community health centres (32) and primary health centres (3). The main intervention was orientation training of doctors and program managers and regular visits to facilities involving assessment, feedback, training and action. The adherence to evidence based practices before, during and after this intervention were measured using structured checklists and scoring sheets. Main outcome measures included changes in practices during labour, delivery or immediate postpartum period.ResultsUse of several unnecessary or harmful practices reduced significantly. Most importantly, proportion of facilities using routine augmentation of labour reduced (p = 0), episiotomy for primigravidas (p = 0.0003), fundal pressure (p = 0.0003), and routine suction of newborns (0 = 0.0005). Among the beneficial practices, use of oxytocin after delivery increased (p = 0.0001) and the practice of listening foetal heart sounds during labour (p = 0.0001). Some practices did not show any improvements, such as dorsal position for delivery, use of partograph, and hand-washing.ConclusionsAn intervention based on repeated facility visits combined with actions at the level of decision makers can lead to substantial improvements in quality of childbirth practices at health facilities.

Race, ethnicity, and cultural attitudes and practices are among the variables that influence health behaviors, including adaptive health behaviors. The following discussions highlight the important role of social conditions in shaping health behaviors and the central role of family in promoting health across the Asian, Hispanic, Native American, and African American ethnic groups. Factors that may lead to health-damaging behaviors are also discussed. The need for additional research that identifies correlations among physiological, social, and behavioral factors and health behaviors, as well as underlying mechanisms, is called for. Keywordsaging; behavior; coping; ethnicity; health Although little is known about the adaptive health behaviors of minority groups, in this article, we nevertheless summarize what is currently known, using both empirical research and theoretical treatises. We address the adaptive health behaviors of four minority groups: Asian Americans, Native Americans, African Americans, and Hispanic Americans. Although there are many similarities, each group presents unique issues related to the study of adaptive health behaviors.Adaptive health behaviors among Asian Americans are discussed with regard to the usefulness of race and ethnicity as variables in the study of behavior. We suggest that Asian cultural diversity needs to be understood by researchers before they can meaningfully engage in health research with this population. This is especially true because cultural expectations and norms shape the adaptive structures within a culture. We use the cultural characteristics of mainland China and their association with health behaviors as an example.The discussion on Native Americans emphasizes the influence of social conditions on their health behaviors. We also focus on the health habits and practices of young Native Americans and their potential effects on future health status. We suggest how to intervene in the lives of Native American adolescents to help alter their responses to health-changing conditions.