Two patients with benign symmetrical lipomatosis (madelung's disease) have been followed for 7 and 9 years. Despite extensive surgical procedures, both patients have developed multiple recurrences. Lipomas and normal adipose tissue from the same patients were compared using chromatographic techniques. The lipomas contained significantly increased triglyceride fractions. Conservative surgical debulking is the recommended treatment.

The effects of ethanol on the total, nonpolar, and polar lipids of whole liver, mitochondria, and microsomes have been evaluated. Differences in the fatty acid composition of various lipid subclasses have been compared in control and ethanol treated mice. On the whole polyunsaturated fatty acids, especially arachidonic (20∶4) and docosahexaenoic (22∶6), were found to decrease. The significance of an enzymatic mechanism vs. a peroxidative mechanism to explain the results is discussed. Decreases also were observed in the ratios of arachidonate/linoleate following ethanol feeding. These changes are thought to be associated with decreases in the activity of the chain elongation‐desaturation system.

I. An alcohol dehydrogenase (alcohol : NAD oxidoreductase, EC (I. I. I) was isolated from the supernatant fraction of mouse liver and purified zo6-fold. 2. A number of aliphatic aldehydes, ranging from acetaldehyde to octadecanal, as well as benzaldehyde and isobutanal, were capable of being reduced by the enzyme. X, values ranged from g-g x IO-* to 2.5 x 10-s M. 3. Cetyl alcohol was slowly oxidized to the aldehyde by the enzyme in the presence of NAD+. However, attempts to oxidize ethanol were unsuccessful. 4. The enzyme showed a pHoptimum at pH 6.8 withirreversibledenaturationoccurring below pH 5 and above pH g.

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A method is described for the analysis of free and bound fatty aldehydes in lipid extracts. By radiolabeling techniques it was shown that the method for measuring free fatty aldehydes when used in the presence of bound fatty aldehydes measures less than 1% of the bound aldehydes. Parameters of time, temperature and optimum acid concentration are reported. A comparison has been made between the present method and other published methods of measuring p‐nitrophenylhydrazones with emphasis on recoveries and reactivities. The present method is suitable for measuring as little as 0.02 μmoles of fatty aldehyde. The method has been applied to the analysis of free and bound aldehydes present in various mouse tissues.