Background: Trichomonas vaginalis infections are greatly connected with the Existence of other STIs (gonorrhea, chlamydia, and Human Immunodeficiency Virus). Aims of study: To investigate the infection rate of Chlamydia trachomatis and Trichomonas vaginalis in women who attended to Al-Zahraa Hospital for Maternity and Children in Al- Najaf city. Methods: A cross-sectional study conducted on 86 women was attended to Al-Zahraa Hospital for Maternity and Children in Al- Najaf city at period between Aprils to September 2018. Cervical samples were taken from women suffering from cervicitis or cervical erosion to detect the Chlamydia trachomatis and Trichomonas vaginalis. Results: the results show there was 50 positive samples with Chlamydia trachomatis (58.2%) and 36 sample negative  (41.8%) and showed there was 24 positive samples with Trichomonas vaginalis (27.9) % and negative was 62  (72.1) % with significant difference (P ≤ 0.05) between positive and negative patients. There was no associated between these microorganisms and use contraceptive or none, mode of delivery and time of the menstrual cycle. Conclusion: This study concludes high percentage of Chlamydia trachomatis with intermediate percentage of Trichomonas vaginalis among women in Al- Najaf city. No significant effect between these organisms and the variables in this study may be indicate the ability of them to spread in any case and required the accurately diagnosed and treated. Recommendation: As a result, the present study recommends the epidemiological study of migratory diseases periodically and increasing the awareness procedures for the damage of these diseases and their risks.