J. 1996. Genetically stable celi lines of cucumber for the large-scale production of diploid somatic embryos. -Physiol. Plant. 97: 303-310.For the initiation of embr\'ogenic cucumber (Cucumis sativus L.) cell lines, from excised radicles, directly in liquid medium, the culture regime, explant density' and type and concentration of hormones were adjusted so that pro-embryogenic masses (PEMs) were formed within about 8 weeks. The established cucumber cell lines were maintained for severai years without loss of embryogenic and genetic stability. The ploidy level of somatic embryos from different cucumber eel! lines was either diploid or tetraploid and depended on the ploidy level of Ihe cell line. Cucumber cell lines that produced only diploid embryos were obtained by selecting completely diploid explant material and growing 11 in the dark during the initiation phase, Mixoploid explants could lead to tetraploid or mixoploid cell lines. Isolation and additional selection and subculturing of single PEMs resulted in either completely diploid or tetraploid eel! lines, indicating that all ceils of individual PEMs are either diploid or tetraploid. The embryogenic cucumber cell lines, differing only in ploidy level, were indistinguishable in growth rate and embrj'ogenic potential and were genetically stable over several years. Kev words -Cucumis sativus, genetic stability, somatic embryogenesis.M. Kreuger (corresponding author,.^48) et aL.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.