Cucurbita ficifolia
Bouché is an important germplasm resource used for rootstock and hypoglycemic food in Cucurbitaceae. The complete chloroplast genome sequence of
C. ficifolia
has been determined in this study. The total genome size is 157,533 bp in length and contains a pair of inverted repeats (IRs) of 25,639 bp, which were separated by large single copy (LSC) and small single copy (SSC) of 88,112 bp and 18,143 bp, respectively. A total of 130 genes were predicted including 86 protein-coding genes, eight rRNA genes and 36 tRNA genes. Further, Maximum-likelihood phylogenetic analysis revealed that
C. ficifolia
is a base clade of genus Cucurbita and closer to Cucurbita maxima. The chloroplast genome of
C. ficifolia
would promote the germplasm exploration, phylogenetic relationships, and molecular biology researches in Cucurbita.
Glaesserella (Haemophilus) parasuis (G. parasuis) is a commensal bacterium in the swine upper respiratory tract that can cause Glässer’s disease, particularly in piglets. In this study, we detected the serovars and 19 known virulence genes (VGs) of H. parasuis isolates from the nasal cavities of live piglets from the south of China.Serovar 10 (17.9%) was the most prevalent, followed by serovars 15 (14.5%), 6 (12.0%), 8 (11.1%), 4 (8.5%), 7 (7.7%), 9 (7.7%), 1 (7%), 5/12 (4.3%), and 2 (0.9%). This differs from previous studies on common G. parasuis serovars. The detection rate of 19 VGs ranged from 1.7% to 95.2%, with vacJ and clpP (95.7%) as the most prevalent. The G. parasuis isolates belonging to the same sequence type and serovar harbored different VGs, and all isolates exhibited considerable genetic heterogeneity. Significant correlations were found between VGs and serovars, different pathogenic serovar groups, and members of clade 2 (based on MLST). To our knowledge, this is the first research to examine the characteristics of G. parasuis nasal cavity isolates from live piglets in the south of China. The results complement epidemiological data of G. parasuis and will help the scientific community understand the extreme genetic diversity and pathogenesis of G. parasuis, which will aid in the development of G. parasuis vaccines.
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