In this paper, a core-shell based on the Fe3O4@SiO2@Au nanoparticle amplification technique for a surface plasmon resonance (SPR) sensor is proposed. Fe3O4@SiO2@AuNPs were used not only to amplify SPR signals, but also to rapidly separate and enrich T-2 toxin via an external magnetic field. We detected T-2 toxin using the direct competition method in order to evaluate the amplification effect of Fe3O4@SiO2@AuNPs. A T-2 toxin–protein conjugate (T2-OVA) immobilized on the surface of 3-mercaptopropionic acid-modified sensing film competed with T-2 toxin to combine with the T-2 toxin antibody–Fe3O4@SiO2@AuNPs conjugates (mAb-Fe3O4@SiO2@AuNPs) as signal amplification elements. With the decrease in T-2 toxin concentration, the SPR signal gradually increased. In other words, the SPR response was inversely proportional to T-2 toxin. The results showed that there was a good linear relationship in the range of 1 ng/mL~100 ng/mL, and the limit of detection was 0.57 ng/mL. This work also provides a new possibility to improve the sensitivity of SPR biosensors in the detection of small molecules and in disease diagnosis.
Based on the gas–liquid phase chemiluminescence tester independently developed by our laboratory, a highly sensitive, fast and accurate on-line detection method of formaldehyde gas in ambient atmosphere is established.
It is an urgent need to determine the concentration of ozone in atmosphere; however, most current methods are limited by large-scale equipments or complex procedures. Herein, a gas-liquid chemiluminescence (GL-CL)...
Landing a helicopter to the ship flight deck is most demanding even for the most experienced pilots and modeling and simulation of the ship-helicopter dynamic interface is a substantially challenging technical problem. In this paper, a coupling numerical method was developed to simulate the fully coupled ship-helicopter flow-field under complete wind-over-deck conditions. The steady actuator disk model based on the momentum source approach and the resolved blade method based on the moving overset mesh method were employed to model the rotor. Two different ship-helicopter combinations were studied. The helicopter flight mechanics model was established and then the influences of coupled airwake on the helicopter were analyzed. Finally, based on the derived rejection criterion of safe landing and the developed numerical method, the flight envelopes for these two ship-helicopter combinations were predicted. The steady actuator disk model was found to be effective in the study of helicopter operations in the shipboard environment. The calculated flight envelopes indicate that an appropriate wind direction angle is beneficial to increasing the allowable maximum wind speed and the operating boundary is affected by the rotation direction of the main rotor.
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